HMGB1/HMG-1 Antibody (19N15F4) [DyLight 488]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27401G

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (99%), Canine (100%), Chicken (91%), Chinese Hamster (99%), Equine (99%), Porcine (99%), Primate (100%), Rat (99%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

DyLight 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 19N15F4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HMGB1/HMG-1 Antibodies »

Product Specifications

Immunogen

Full-length recombinant human HMGB1 was used as the immunogen for the antibody.

Reactivity Notes

Based upon immunogen sequence similarity, this antibody is predicted to react 100% with olive baboon, crab-eating macaque, and common marmoset.

Localization

Nucleus (very high, ubiquitous), Cytoplasmic (very low, some tissues)

Specificity

This antibody recognizes both the box A domain (amino acids 9-79) and full length HMGB1 protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Applications for HMGB1/HMG-1 Antibody (19N15F4) [DyLight 488]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: HMGB1/HMG-1

HMGB1 and HMGB2 are part of the chromatin non-histone high mobility group proteins 1 and 2. HMGB1 and HMGB2 (containing multiple HMG-boxes) are conserved domains of 80 amino acids which mediate the DNA binding of many proteins. HMG box domains recognize DNA structure. Both HMGB1 and HMGB2 contain a N-terminal HMG box, a central HMG box, and an acidic carboxy terminus. The acidic tails of HMGB1 and HMGB2 contain multiple serine residues which match the phosphorylation consensus sites of casein kinase II, and phosphorylation of this domain appears to be important for proper functioning of these proteins. HMGB1 and HMGB2 have been shown to facilitate the binding of various sequence-specific transcription factors to their respective DNA binding sites. HMGB1 and HMGB2 may also serve as architectural factors that recognize and mediate DNA structural changes that accompany various events such as DNA repair, transcription, and replication.

Long Name

High Mobility Group Protein 1

Alternate Names

HMG-1, HMG1

Gene Symbol

HMGB1

Additional HMGB1/HMG-1 Products

Product Documents for HMGB1/HMG-1 Antibody (19N15F4) [DyLight 488]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HMGB1/HMG-1 Antibody (19N15F4) [DyLight 488]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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