Antibody was raised against a 19 amino acid peptide near the center of human HMGB1. The immunogen is located within amino acids 70 - 120 of HMGB1. Amino Acid Squence: KGETKKKFKDPNAPKRPPS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HMGB1/HMG-1 Antibody - BSA Free

Western Blot: HMGB1/HMG-1 AntibodyBSA Free [NBP2-41264]

Western Blot: HMGB1/HMG-1 Antibody [NBP2-41264] - Western blot analysis of HMGB1 in human brain tissue lysate with HMGB1 antibody at (A) 1 and (B) 2 ug/ml

Immunocytochemistry/ Immunofluorescence: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264]

Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 Antibody [NBP2-41264] - HMGB1/HMG-1 in human lung tissue with HMGB1/HMG-1 antibody at 20 ug/mL.

Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264]

Immunohistochemistry: HMGB1/HMG-1 Antibody [NBP2-41264] - Immunohistochemistry of HMGB1/HMG-1 in human lung tissue with HMGB1 antibody at 5 ug/mL.

Immunocytochemistry/ Immunofluorescence: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] -

Immunocytochemistry/ Immunofluorescence: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Figure 5 Immunofluorescence Validation of HMGB1/HMG-1 in HL60 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed HL60 cells labeling HMGB1/HMG-1 with at 20 u /mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).

Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] -

Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Figure 3 WB Validation in Mouse TissuesLoading: 15 u of lysate Antibodies: HMGB1/HMG-1 , 1 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Knockout Validated: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] -

Knockout Validated: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Figure 1 KO Validation in HeLa Cells
Loading: 10 u of HeLa WT cell lysates or HMGB1/HMG-1 KO cell lysates. Antibodies: HMGB1/HMG-1 (0.5 u/mL) and beta-actin 3779 (1 u/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] -

Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Figure 6 Immunohistochemistry Validation of HMGB1/HMG-1 in Human Stomach Tissue Immunohistochemical analysis of paraffin-embedded human stomach tissue using anti-HMGB1/HMG-1 antibody at 1 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] -

Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Figure 7 Immunohistochemistry Validation of HMGB1/HMG-1 in Mouse Spleen Tissue
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-HMGB1/HMG-1 antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunocytochemistry/ Immunofluorescence: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] -

Immunocytochemistry/ Immunofluorescence: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Figure 5 Immunofluorescence Validation of HMGB1/HMG-1 in HL60 Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed HL60 cells labeling HMGB1/HMG-1 with at 20 u /mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).

Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] -

Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Figure 7 Immunohistochemistry Validation of HMGB1/HMG-1 in Mouse Spleen Tissue Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-HMGB1/HMG-1 antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Knockout Validated: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] -

Knockout Validated: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Figure 1 KO Validation in HeLa Cells Loading: 10 u of HeLa WT cell lysates or HMGB1/HMG-1 KO cell lysates. Antibodies: HMGB1/HMG-1 (0.5 u/mL) and beta-actin (1 u/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] -

Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Figure 4 WB Validation in Rat Tissues Loading: 15 u of lysate Antibodies: HMGB1/HMG-1 , 1 u/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] -

Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Figure 4 WB Validation in Rat Tissues
Loading: 15 u of lysate Antibodies: HMGB1/HMG-1 , 1 u/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] -

Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Figure 3 WB Validation in Mouse Tissues
Loading: 15 u of lysate Antibodies: HMGB1/HMG-1 , 1 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] -

Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Figure 2 WB Validation in Human, Mouse and Rat Cell Lines Loading: 15 u of lysate Antibodies: HMGB1/HMG-1 , 1 u/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] -

Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Figure 2 WB Validation in Human, Mouse and Rat Cell Lines
Loading: 15 u of lysate Antibodies: HMGB1/HMG-1 , 1 u/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] -

Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Figure 8 Immunohistochemistry Validation of HMGB1/HMG-1 in Rat Spleen Tissue Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-HMGB1/HMG-1 antibody at 1 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] -

Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Figure 8 Immunohistochemistry Validation of HMGB1/HMG-1 in Rat Spleen Tissue
Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-HMGB1/HMG-1 antibody at 1 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] -

Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Figure 6 Immunohistochemistry Validation of HMGB1/HMG-1 in Human Stomach Tissue
Immunohistochemical analysis of paraffin-embedded human stomach tissue using anti-HMGB1/HMG-1 antibody at 1 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

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Applications for HMGB1/HMG-1 Antibody - BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

20ug/mL

Immunohistochemistry

1-2 ug/mL

Western Blot

1 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HMGB1/HMG-1

HMGB1 and HMGB2 are part of the chromatin non-histone high mobility group proteins 1 and 2. HMGB1 and HMGB2 (containing multiple HMG-boxes) are conserved domains of 80 amino acids which mediate the DNA binding of many proteins. HMG box domains recognize DNA structure. Both HMGB1 and HMGB2 contain a N-terminal HMG box, a central HMG box, and an acidic carboxy terminus. The acidic tails of HMGB1 and HMGB2 contain multiple serine residues which match the phosphorylation consensus sites of casein kinase II, and phosphorylation of this domain appears to be important for proper functioning of these proteins. HMGB1 and HMGB2 have been shown to facilitate the binding of various sequence-specific transcription factors to their respective DNA binding sites. HMGB1 and HMGB2 may also serve as architectural factors that recognize and mediate DNA structural changes that accompany various events such as DNA repair, transcription, and replication.

Long Name

High Mobility Group Protein 1

Alternate Names

HMG-1, HMG1

Gene Symbol

HMGB1

Additional HMGB1/HMG-1 Products

Product Documents for HMGB1/HMG-1 Antibody - BSA Free

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for HMGB1/HMG-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

HMGB1/HMG-1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-41264

Key Product Details

Validated by

Species Reactivity

Validated:

Predicted:

Applications

Label

Antibody Source

Format

Concentration

View all HMGB1/HMG-1 Antibodies »

Product Specifications

Immunogen

Clonality

Host

Isotype

Theoretical MW

Scientific Data Images for HMGB1/HMG-1 Antibody - BSA Free

Western Blot: HMGB1/HMG-1 AntibodyBSA Free [NBP2-41264]

Immunocytochemistry/ Immunofluorescence: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264]

Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264]

Immunocytochemistry/ Immunofluorescence: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] -

Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] -

Knockout Validated: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] -

Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] -

Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] -

Immunocytochemistry/ Immunofluorescence: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] -

Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] -

Knockout Validated: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] -

Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] -

Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] -

Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] -

Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] -

Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] -

Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] -

Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] -

Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] -

Applications for HMGB1/HMG-1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: HMGB1/HMG-1

Long Name

Alternate Names

Gene Symbol

Additional HMGB1/HMG-1 Products

Product Documents for HMGB1/HMG-1 Antibody - BSA Free

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for HMGB1/HMG-1 Antibody - BSA Free