HNF-3 beta/FoxA2 Antibody (OTI3C10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02088

Novus Biologicals, part of Bio-Techne
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NBP2-02088
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3C10

Concentration

1 mg/ml

View all HNF-3 beta/FoxA2 Antibodies »

Product Specifications

Immunogen

Recombinant protein expressed in E.coli corresponding to amino acids 300-458 of human FOXA2

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

48.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HNF-3 beta/FoxA2 Antibody (OTI3C10)

Western Blot: HNF-3 beta/FoxA2 Antibody (OTI3C10) [NBP2-02088]

Western Blot: HNF-3 beta/FoxA2 Antibody (OTI3C10) [NBP2-02088]

Western Blot: HNF-3 beta/FoxA2 Antibody (OTI3C10) [NBP2-02088] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti HNF-3 beta/FoxA2 monoclonal antibody.(1:200).
Immunocytochemistry/ Immunofluorescence: HNF-3 beta/FoxA2 Antibody (OTI3C10) [NBP2-02088]

Immunocytochemistry/ Immunofluorescence: HNF-3 beta/FoxA2 Antibody (OTI3C10) [NBP2-02088]

Immunocytochemistry/Immunofluorescence: HNF-3 beta/FoxA2 Antibody (OTI3C10) [NBP2-02088] - Staining of HepG2 cells using anti HNF-3 beta/FoxA2 mouse monoclonal antibody at 1:50 dilution.
Immunohistochemistry-Paraffin: HNF-3 beta/FoxA2 Antibody (OTI3C10) [NBP2-02088]

Immunohistochemistry-Paraffin: HNF-3 beta/FoxA2 Antibody (OTI3C10) [NBP2-02088]

Immunohistochemistry-Paraffin: HNF-3 beta/FoxA2 Antibody (OTI3C10) [NBP2-02088] - Staining of paraffin-embedded lung using anti-FOXA2 mouse monoclonal antibody.
View 4 more images

Applications for HNF-3 beta/FoxA2 Antibody (OTI3C10)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HNF-3 beta/FoxA2

FOXA2 is a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific genes such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Transcript variants encoding different isoforms have been identified for this gene

Long Name

Hepatocyte Nuclear Factor-3, beta

Alternate Names

FoxA2, HNF3 beta

Entrez Gene IDs

3170 (Human)

Gene Symbol

FOXA2

Additional HNF-3 beta/FoxA2 Products

Product Documents for HNF-3 beta/FoxA2 Antibody (OTI3C10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HNF-3 beta/FoxA2 Antibody (OTI3C10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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