Human 17 beta-HSD1/HSD17B1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7178
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human 17 beta-HSD1/HSD17B1
Ala2-Gln328
Accession # P14061
Ala2-Gln328
Accession # P14061
Specificity
Detects human 17 beta-HSD1/HSD17B1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human 17 beta-HSD1/HSD17B1 Antibody
Detection of Human 17 beta-HSD1/HSD17B1 by Western Blot.
Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human 17 beta-HSD1/HSD17B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7178) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for 17 beta-HSD1/HSD17B1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
17 beta‑HSD1/HSD17B1 in Human Alzheimer's Brain.
17 beta-HSD1/HSD17B1 was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Sheep Anti-Human 17 beta-HSD1/HSD17B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7178) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Human 17 beta-HSD1/HSD17B1 by Simple WesternTM.
Simple Western lane view shows lysates of human placenta, loaded at 0.2 mg/mL. A specific band was detected for 17 beta-HSD1/HSD17B1 at approximately 40 kDa (as indicated) using 2.5 µg/mL of Sheep Anti-Human 17 beta-HSD1/HSD17B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7178) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human 17 beta-HSD1/HSD17B1 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human Alzheimer's brain
Sample: Immersion fixed paraffin-embedded sections of human Alzheimer's brain
Simple Western
2.5 µg/mL
Sample: Human placenta
Sample: Human placenta
Western Blot
0.2 µg/mL
Sample: Human placenta tissue
Sample: Human placenta tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: 17 beta-HSD1/HSD17B1
HSD17B1 (Hydroxysteroid 17-beta dehydrogenase type 1; also 17 beta‑HSD1 and E2DH) is a 33-34 kDa member of the short chain dehydrogenase/reductase (SDR) superfamily of molecules. It is a cytoplasmic breast, placental and gonadal enzyme that both converts DHEA to estrogen A-diol, and inactivates DHT through oxidation and reduction. Human HSD17B1 is 328 amino acids (aa) in length. It contains an NADP binding site (aa 10-38) and a steroid catalytic site (aa 210-221). HSD17B1 functions as a 68 kDa noncovalently-linked homodimer. Over aa 2-328, human HSD17B1 shares 65% aa identity with mouse HSD17B1.
Long Name
17 beta Hydroxysteroid Dehydrogenase 1
Alternate Names
17 betaHSD1, E2DH, EDH17B2, EDHB17, HSD17B1, SDR28C1
Gene Symbol
HSD17B1
UniProt
Additional 17 beta-HSD1/HSD17B1 Products
Product Documents for Human 17 beta-HSD1/HSD17B1 Antibody
Product Specific Notices for Human 17 beta-HSD1/HSD17B1 Antibody
For research use only
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