Human AAK1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6886

R&D Systems, part of Bio-Techne
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MAB6886
MAB6886-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 702425

View all AAK1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human AAK1
Ser704-Ile822
Accession # Q2M2I8

Specificity

Detects human AAK1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human AAK1 Antibody

Detection of Human and Mouse AAK1 antibody by Western Blot.

Detection of Human and Mouse AAK1 by Western Blot.

Western blot shows lysates of IMR-32 human neuroblastoma cell line, Neuro-2A mouse neuroblastoma cell line, and L1.2 mouse pro-B cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human AAK1 Monoclonal Antibody (Catalog # MAB6886) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for AAK1 (Long Form) at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human AAK1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: IMR‑32 human neuroblastoma cell line, Neuro‑2A mouse neuroblastoma cell line, and L1.2 mouse pro-B cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AAK1

AP2-associated protein kinase 1 (AAK1) is a 100 kDa intracellular kinase that binds and phosphorylates NUMB and the m2 subunit of AP2. NUMB and AP2 are adaptor proteins which are involved in the assembly and sorting of clathrin coated pits during receptor-mediated endocytosis. AAK1 contains one catalytic domain (aa 46‑315) and a C-terminal clathrin-binding region. Alternate splicing of human AAK1 generates a 145 kDa long isoform that contains an additional clathrin-binding domain. Within aa 704‑822, human AAK1 shares 95% aa sequence identity with mouse and rat AAK1.

Long Name

AP2-associated Protein Kinase 1

Alternate Names

Adaptor-associated kinase 1, AP2 associated kinase 1, AP2-associated protein kinase 1, DKFZp686F03202, DKFZp686K16132, EC 2.7.11.1, FLJ23712, FLJ25931, FLJ31060, FLJ45252, KIAA1048FLJ42882, MGC138170, MGC164568, MGC164570

Entrez Gene IDs

22848 (Human); 269774 (Mouse); 500244 (Rat)

Gene Symbol

AAK1

UniProt

Q2M2I8

Additional AAK1 Products

Product Documents for Human AAK1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human AAK1 Antibody

For research use only

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