Human ABI1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10249
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1013203
Product Specifications
Immunogen
E. coli-derived recombinant human ABI1
Val429-Asp508
Accession # Q8IZP0
Val429-Asp508
Accession # Q8IZP0
Specificity
Detects human ABI1 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human ABI1 Antibody
ABI1 in A549 Human Cell Line.
ABI1 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human ABI1 Monoclonal Antibody (Catalog # MAB10249) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
ABI1 in Human Brain Tissue.
ABI1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) tissue using Mouse Anti-Human ABI1 Monoclonal Antibody (Catalog # MAB10249) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human ABI1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-25 µg/mL
Sample: Immersion fixed A549 human lung carcinoma cell line
Sample: Immersion fixed A549 human lung carcinoma cell line
Immunohistochemistry
1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex) tissue
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex) tissue
Reviewed Applications
Read 1 review rated 5 using MAB10249 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ABI1
References
- UniProt: The Universal Protein Knowledgebase. (2017) Nucleic Acids Res. 45: D158-D169.
- Fan. P.-D. and S.P. Goff (2000) Mol. Cell. Biol. 20:7591.
- Xiong X. et al. (2008) Biochim Biophy. Acta 1783:737.
Long Name
Abl Interactor 1
Alternate Names
ABI-1, ABLBP4, E3B1, NAP1BP, SSH3BP, SSH3BP1
Entrez Gene IDs
10006 (Human)
Gene Symbol
ABI1
UniProt
Additional ABI1 Products
Product Documents for Human ABI1 Antibody
Product Specific Notices for Human ABI1 Antibody
For research use only
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