Human Activin RIB/ALK-4 Alexa Fluor® 594-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB2221T
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Alexa Fluor 594 (Excitation = 590 nm, Emission = 617 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 544310
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Activin RIB/ALK‑4
Ser24-Glu126
Accession # P36896
Ser24-Glu126
Accession # P36896
Specificity
Detects human Activin RIB/ALK‑4 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Activin RIA, RIIA, or RIIB is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human Activin RIB/ALK-4 Alexa Fluor® 594-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
0.25-1 µg/106 cells
Sample: PC-3 human prostate cancer cell line
Sample: PC-3 human prostate cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Activin RIB/ALK-4
Activin RIB, also known as ALK4, is a glycosylated 58 kDa type I receptor in the superfamily of TGF-beta serine/threonine kinase receptors. Activin RIB associates with Activin RIIB to form a receptor complex for activin and inhibin molecules (1). These ligands bind to Activin RIIB which then associates with and phosphorylates the cytoplasmic domain of Activin RIB to initiate signal transduction (2, 3). Mature human Activin RIB consists of a 103 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 356 aa cytoplasmic region that includes the kinase domain (4). Within the ECD, human Activin RIB shares 93% and 95% aa sequence identity with mouse and rat Activin RIB, respectively. It shares 25-35% aa sequence identity with other human type I receptors Activin RIA, Activin RIC, BMPR-IA, BMPR-IB, and TGF-beta R1. Alternately spliced isoforms of Activin RIB have deletions in the cytoplasmic domain and function as dominant negative inhibitors of activin signaling (5, 6). Activin receptor signaling is modulated by the direct interaction of Activin RIB with cripto or inhibin binding protein (7-9). Activin RIB is excluded from the signaling complex if Activin RIIB first binds inhibin and betaglycan (10). Activin RIB functions in a wide variety of growth and differentiation processes, including embryonic cell fate and axis determination, cell proliferation and apoptosis, and tumorigenesis (1, 11, 12).
References
- Chen, Y- G. et al. (2006) Exp. Biol. Med. 231:534.
- Attisano, L. et al. (1996) Mol. Cell. Biol. 16:1066.
- Tsuchida, K. et al. (1995) Endocrinology 136:5493.
- ten Dijke, P. et al. (1993) Oncogene 8:2879.
- Xu, J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:7957.
- Zhou, Y. et al. (2000) Mol. Endocrinol. 14:2066.
- Chapman, S.C. and T.K. Woodruff (2001) Mol. Endocrinol. 15:668.
- Bianco, C. et al. (2002) Mol. Cell. Biol. 22:2586.
- Gray, P.C. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5193.
- Lewis, K.A. et al. (2000) Nature 404:411.
- Gu, Z. et al. (1998) Genes Dev. 12:844.
- Chen, Y. et al. (2004) Dev. Biol. 268:280.
Long Name
Activin Receptor IB
Alternate Names
ActivinRIB, ACVR1B, ALK-4, ALK4
Gene Symbol
ACVR1B
UniProt
Additional Activin RIB/ALK-4 Products
Product Documents for Human Activin RIB/ALK-4 Alexa Fluor® 594-conjugated Antibody
Product Specific Notices for Human Activin RIB/ALK-4 Alexa Fluor® 594-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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