Human ADAM15 Ectodomain Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB935
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
CyTOF-ready, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Intracellular Staining by Flow Cytometry, Western Blot
Cited:
Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 23G9
Product Specifications
Immunogen
COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant human ADAM15
Asp207-Thr696
Accession # Q13444
Asp207-Thr696
Accession # Q13444
Specificity
Detects the ectodomain of recombinant human (rh) ADAM15 in direct ELISAs and Western blots. In direct ELISAs, shows 100% cross-reactivity with recombinant mouse (rm) ADAM15 but does not cross-react with rhADAM8, 9, 17, 28, rmADAM9, or 10.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human ADAM15 Ectodomain Antibody
ADAM15 in MCF‑7 Human Cell Line.
ADAM15 was detected in immersion fixed MCF-7 human breast cancer cell line using Human ADAM15 Ectodomain Monoclonal Antibody (Catalog # MAB935) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
ADAM15 in Human Prostate Cancer Tissue.
ADAM15 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 25 µg/mL Human ADAM15 Ectodomain Monoclonal Antibody (Catalog # MAB935) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of ADAM15 in PC-3 cells (Positive) & MOLT-4 cells (Negative).
ADAM15 was detected in immersion fixed PC-3 human prostate cancer cells (Positive) & absent in MOLT-4 human acute lymphoblastic leukemia cells (Negative) using Mouse Anti-Human ADAM15 Ectodomain Monoclonal Antibody (Catalog # MAB935) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to Cytoplasmic. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human ADAM15 Ectodomain Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line, PC-3 human prostate cancer cells (Positive) & absent in MOLT-4 human acute lymphoblastic leukemia cells (Negative)
Sample: Immersion fixed MCF-7 human breast cancer cell line, PC-3 human prostate cancer cells (Positive) & absent in MOLT-4 human acute lymphoblastic leukemia cells (Negative)
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human ADAM15, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human ADAM15, see our available Western blot detection antibodies
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: MCF-7 human breast cancer cell line fixed with paraformaldehyde and permeabilized with saponin
Sample: MCF-7 human breast cancer cell line fixed with paraformaldehyde and permeabilized with saponin
Western Blot
1 µg/mL
Sample: Recombinant Human ADAM15 Western Blot Standard (Catalog # WBC027)
under non-reducing conditions only
Sample: Recombinant Human ADAM15 Western Blot Standard (Catalog # WBC027)
under non-reducing conditions only
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using MAB935 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ADAM15
Long Name
A Disintegrin and Metalloprotease-like Domain 15
Alternate Names
MDC15, Metargidin
Gene Symbol
ADAM15
UniProt
Additional ADAM15 Products
Product Documents for Human ADAM15 Ectodomain Antibody
Product Specific Notices for Human ADAM15 Ectodomain Antibody
For research use only
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