Human ADAM32 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5380
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ADAM32
Ser17-Thr476
Accession # Q8TC27
Ser17-Thr476
Accession # Q8TC27
Specificity
Detects human ADAM32 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) ADAM9, rhADAM22, and rhADAM23 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human ADAM32 Antibody
Detection of Human ADAM32 by Western Blot.
Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ADAM32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5380) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ADAM32 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human ADAM32 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human ADAM32, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human ADAM32, see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Human testis tissue
Sample: Human testis tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ADAM32
Long Name
A Disintegrin and Metalloproteinase Domain 32
Alternate Names
a disintegrin and metalloprotease domain 32, a disintegrin and metalloproteinase domain 32, ADAM 32, ADAM metallopeptidase domain 32, disintegrin and metalloproteinase domain-containing protein 32, FLJ26299, FLJ29004, metalloproteinase 12-like protein
Gene Symbol
ADAM32
UniProt
Additional ADAM32 Products
Product Documents for Human ADAM32 Antibody
Product Specific Notices for Human ADAM32 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...