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Human Adenosine A2aR/A2bR Alexa Fluor® 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB94973G

R&D Systems, part of Bio-Techne
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FAB94973G-100UG

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 826037

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human Adenosine A2aR
Met1-Ser412
Accession # P29274

Specificity

Detects human Adenosine A2aR in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications

Application
Recommended Usage

Flow Cytometry

Titration recommended for optimal concentration with starting range of 0.1-1 µg/1 million cells. Sample used for this experiment was HEK293 cells transfected with Human ADORA-2A and eGFP.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Adenosine A2aR/A2bR

Adenosine A2A Receptor (ADORA2A, Adenosine A2aR) is one of several receptor subtypes for adenosine. The activity ofAdenosine A2aR, a G protein-coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. Adenosine A2aR is abundant in basal ganglia, vasculature and platelets and is a major target of caffeine. Variability in the acute blood pressure response to coffee may be partly explained by genetic polymorphisms of the adenosine A2A receptors, including Adenosine A2aR, and alpha(2)-adrenergic receptors.

Long Name

Adenosine A2A Receptor/Adenosine A2B Receptor

UniProt

Additional Adenosine A2aR/A2bR Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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