HEK293 cells transfected with Human ADORA-2A and eGFP were stained with eGFP and either (A) Mouse Anti-Human Adenosine A2aR/A2bR Monoclonal Antibody (Catalog # MAB94973) or (B) Mouse IgG_2B Isotype Control (Catalog # MAB004) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Adenosine A2aR/A2bR Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 cells transfected with Human ADORA-2A and eGFP

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Adenosine A2aR/A2bR

Adenosine is a ubiquitous endogenous molecule that affects almost all aspects of cellular physiology. The four Adenosine G protein-coupled receptors (GPCR) A₁, A_2A, A_2B, and A₃ have been described as key metabolic and immune-checkpoint regulators implicated in the tumor escape from the host immune system becoming both, markers of pathologies, and useful targets for novel drugs. Adenosine receptors A_2A and A_2B (also known as ADORA2A and ADORA2B, respectively) have been also shown to play an important cardio-protective role.

Long Name

Adenosine A2A Receptor/Adenosine A2B Receptor

UniProt

P29274

Additional Adenosine A2aR/A2bR Products

Product Documents for Human Adenosine A2aR/A2bR Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Adenosine A2aR/A2bR Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Adenosine A2aR/A2bR Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB94973

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all Adenosine A2aR/A2bR Antibodies »

Product Summary for Human Adenosine A2aR/A2bR Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Adenosine A2aR/A2bR Antibody

Detection of Adenosine A2aR/A2bR in HEK293 cells transfected with Human ADORA-2A and eGFP cells by Flow Cytometry.