Human Adenosylhomocysteinase/AHCY Antibody

R&D Systems, part of Bio-Techne | Catalog # AF6466

R&D Systems, part of Bio-Techne
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AF6466
AF6466-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Adenosylhomocysteinase/AHCY Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Adenosylhomocysteinase/AHCY
Ser2-Tyr432
Accession # P23526

Specificity

Detects human Adenosylhomocysteinase/AHCY in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Adenosylhomocysteinase/AHCY Antibody

Detection of Human Adenosylhomocysteinase/AHCY antibody by Western Blot.

Detection of Human Adenosylhomocysteinase/AHCY by Western Blot.

Western blot shows lysates of Bowes human melanoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human Adenosylhomocysteinase/AHCY Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6466) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Adenosylhomocysteinase/AHCY at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of Human Adenosylhomocysteinase/AHCY by Simple WesternTM.

Simple Western shows lysates of Exosome Standards (HT-29) (NBP3-11685), Exosome Standards (PC-3) (NBP2-49856) and K562 human chronic myelogenous leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for Adenosylhomocysteinase/AHCY at approximately 50 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Adenosylhomocysteinase/AHCY Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6466). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Applications for Human Adenosylhomocysteinase/AHCY Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: Exosome Standards (HT-29) (Catalog # NBP3-11685), Exosome Standards (PC-3) (Catalog # NBP2-49856) and K562 human chronic myelogenous leukemia cell line

Western Blot

1 µg/mL
Sample: Bowes human melanoma cell line and HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Adenosylhomocysteinase/AHCY

Human S‑Adenosylhomocysteinase (AHCY) is a cytoplasmic tetramer with a tightly bound NAD co‑factor for each subunit (1, 2). It is the only known enzyme to catalyze the breakdown of S‑adenosylhomocysteine (AdoHcy) to homocysteine and adenosine. AdoHcy hydrolysis is a reversible reaction with an equilibrium favoring AdoHcy formation, but hydrolysis prevails under physiological conditions due to the rapid removal of adenosine and homocysteine. Thus, AHCY’s activity in mammals is directly related to homocysteine level, an independent risk factor for vascular disease (3). It also functions as a regulator of biological transmethylation by controlling the concentration of AdoHcy, a potent competitive inhibitor of all S‑adonosyl-L-methionine methyltransferases (1). A mutation in the human AHCY results in AHCY deficiency with increase of plasma creatine kinase, methionine, S‑adenosylmethionine and AdoHcy, delay of myelination, myopathy and psychomotor retardation (4, 5).

References

  1. Turner, M. A. et al. (2000) Cell Biochem. Biophys. 33:101.
  2. Takata, Y. et al. (2002) J. Biol. Chem. 277:22670.
  3. Gellekink, H. et al. (2004) Eur. J. Hum. Genet. 12:942.
  4. Baric, I. et al. (2004) Proc. Natl. Acad. Sci. USA. 101:4234.
  5. Fumic, K. et al. (2007) Eur. J.Hum. Genet. 15:347. 

Alternate Names

AdoHcyase, AHCY, SAHH

Entrez Gene IDs

191 (Human)

Gene Symbol

AHCY

UniProt

P23526 (Human)

Additional Adenosylhomocysteinase/AHCY Products

Product Documents for Human Adenosylhomocysteinase/AHCY Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Adenosylhomocysteinase/AHCY Antibody

For research use only

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