HEK293 human embryonic kidney cell line transfected with (A) human Adrenomedullin R/ADMR or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Adrenomedullin R/ADMR Monoclonal Antibody (Catalog # MAB10293) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Adrenomedullin R/ADMR in Human Spleen Tissue.

Adrenomedullin R/ADMR was detected in immersion fixed paraffin-embedded sections of human spleen tissue using Mouse Anti-Human Adrenomedullin R/ADMR Monoclonal Antibody (Catalog # MAB10293) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cell in sinusoids. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Adrenomedullin R/ADMR Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human Adrenomedullin R/ADMR and eGFP

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spleen tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Adrenomedullin R/ADMR

ADMR (GPR182) is a seven transmembrane orphan receptor originally thought to be a receptor for adrenomedullin. ADMR is highly expressed in heart, skeletal muscle, immune system, adrenal gland and liver. Expression is in endothelial cells along the vascular tree.

Long Name

Adrenomedullin Receptor

Alternate Names

7TMR, ADMR, AdrenomedullinR, AMR, G10D, GAMRH, Gpcr22, GPR182, MB10, NOW

Entrez Gene IDs

11318 (Human); 11536 (Mouse); 29307 (Rat)

Gene Symbol

GPR182

UniProt

O15218 (Human)

Additional Adrenomedullin R/ADMR Products

Product Documents for Human Adrenomedullin R/ADMR Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Adrenomedullin R/ADMR Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Adrenomedullin R/ADMR Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10293

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Adrenomedullin R/ADMR Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Adrenomedullin R/ADMR Antibody

Detection of Adrenomedullin R/ADMR in HEK293 Human Cell Line Transfected with Human Adrenomedullin R/ADMR and eGFP by Flow Cytometry.