Human AHR Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6185
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Immunocytochemistry, Intracellular Staining by Flow Cytometry, Knockout Validated, Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human AHR
Asn704-Leu848
Accession # P35869
Asn704-Leu848
Accession # P35869
Specificity
Detects human AHR in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human AHR Antibody
Detection of Human AHR by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and PC-3 human prostate cancer cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human AHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6185) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for AHR at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
AHR in HeLa Human Cell Line.
AHR was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human AHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6185) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of AHR in HeLa Human Cell Line by Flow Cytometry.
HeLa human cervical epithelial carcinoma cell line was stained with Sheep Anti-Human AHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6185, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.Applications for Human AHR Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line fixed with paraformaldehyde and permeabilized with saponin
Sample: HeLa human cervical epithelial carcinoma cell line fixed with paraformaldehyde and permeabilized with saponin
Knockout Validated
AHR is
specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in AHR
knockout HeLa cell line.
Simple Western
10 µg/mL
Sample: PC‑3 human prostate cancer cell line
Sample: PC‑3 human prostate cancer cell line
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and PC‑3 human prostate cancer cell line
Sample: HeLa human cervical epithelial carcinoma cell line and PC‑3 human prostate cancer cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: AHR
AHR (Aryl-hydrocarbon receptor; also known as bHLHE76) is a 110 kDa member of the bHLH/PAS transcription factor family. It is widely expressed (breast, lung, liver), and serves many functions. First, it binds multiple xenobiotic chemicals in the cytoplasm. This induces dimerization with ARNT, translocation to the nucleus, and activation of P450 genes such as CYP1A1 and UGT1A6. Second, it appears to block cell cycle progression, possibly via a down‑regulation of CDK proteins. And third, it blocks apoptosis by interacting with E2F1, thus silencing TP73 and Apaf1 genes. Human AHR is 848 amino acids (aa) in length. It contains a 10 aa prosegment, plus a 838 aa mature molecule that contains a DNA binding motif (aa 13-40), a bHLH region (aa 41-81), and two PAS domains (aa 111-342). Over aa 704-848, human AHR shares 70% aa identity with mouse AHR.
Long Name
Aryl Hydrocarbon Receptor
Alternate Names
BHLHE76
Gene Symbol
AHR
UniProt
Additional AHR Products
Product Documents for Human AHR Antibody
Product Specific Notices for Human AHR Antibody
For research use only
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