Human alpha‑N‑acetylgalactosaminidase/NAGA Antibody

R&D Systems, part of Bio-Techne | Catalog # AF6717

R&D Systems, part of Bio-Techne
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AF6717
AF6717-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all alpha-N-acetylgalactosaminidase/NAGA Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line recombinant human alpha‑N‑acetylgalactosaminidase/NAGA
Leu18-Gln411
Accession # P17050

Specificity

Detects human alpha‑N‑acetylgalactosaminidase/NAGA in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human alpha-Galactosidase A is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human alpha‑N‑acetylgalactosaminidase/NAGA Antibody

Detection of Human a-N-acetylgalactosaminidase/NAGA antibody by Western Blot.

Detection of Human alpha‑N‑acetylgalactosaminidase/NAGA by Western Blot.

Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human a-N-acetylgalactosaminidase/NAGA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6717) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for a-N-acetylgalactosaminidase/NAGA at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human alpha‑N‑acetylgalactosaminidase/NAGA Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human placenta tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-N-acetylgalactosaminidase/NAGA

NAGA is a lysosomal alpha-N-acetylgalactosaminidase that cleaves non-reducing alpha-N-acetylgalactosaminyl moieties from glycoconjugates (1). Mature NAGA has 394 amino acids and is trafficked to the lysosome via the mannose-6-phosphate receptor‑mediated pathway (2). The enzyme is a retaining exoglycosidase, where both the substrate and product of the enzymatic reaction have the same anomeric configuration (3). Deficiency in NAGA results in increased urinary excretion and tissue accumulation of glycopeptides and oligosaccharides containing terminal alpha‑N‑acetylgalactosaminyl moieties (4), manifesting as Schindler’s disease, an autosomal recessive disease with neuroaxonal dystrophy and other neurological symptoms (5). The enzyme can be used to remove alpha‑N‑acetylgalactosaminyl residues present on red blood cells thus converting blood type A to blood type O (6, 7, 8).

References

  1. Wang, A.M. et al. (1990) J. Biol. Chem. 265:21859.
  2. Sweeley, C.C. et al. (1983) Arch. Biochem. Biophys. 223:158.
  3. Garman, S.C. et al. (2002) Structure. 10:425.
  4. Eng, C.M. et al. (2001) N. Engl. J. Med. 345:9.
  5. Wang, A.M. et al. (1990) J. Clin. Invest. 86:1752.
  6. Liu, Q.P. et al. (2007) Nature Biotechnol. 25:454.
  7. Calcutt, M. J. et al. (2002) FEMS Microbiol. Lett. 214:77.
  8. Zhu, A. et al. (1996) Arch. Biochem. Biophys. 327:324.

Alternate Names

alphaNacetylgalactosaminidase, NAGA

Entrez Gene IDs

4668 (Human); 17939 (Mouse); 315165 (Rat)

Gene Symbol

NAGA

UniProt

P17050

Additional alpha-N-acetylgalactosaminidase/NAGA Products

Product Documents for Human alpha‑N‑acetylgalactosaminidase/NAGA Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human alpha‑N‑acetylgalactosaminidase/NAGA Antibody

For research use only

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