Human alpha-Taxilin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5575
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human alpha-Taxilin
Met1-Lys162
Accession # P40222
Met1-Lys162
Accession # P40222
Specificity
Detects human alpha-Taxilin in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human alpha-Taxilin Antibody
Detection of Human alpha-Taxilin by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human a-Taxilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5575) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for a-Taxilin at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
alpha‑Taxilin in HepG2 Human Cell Line.
a-Taxilin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Sheep Anti-Human a-Taxilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5575) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human alpha-Taxilin Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line
Western Blot
1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha-Taxilin
alpha‑Taxilin (TXLN‑ alpha; also p75 [in rat] and IL‑14 alpha) is the founding member of the taxilin family of proteins. Although its predicted MW is 62 kDa, it runs anomalously at 74‑76 kDa in SDS‑PAGE. alpha‑Taxilin is ubiquitously expressed and participates in intracellular vesicle trafficking. In particular, alpha‑Taxilin interacts with syntaxin family members that localize to the plasma membrane, providing for SNARE complex formation. alpha‑Taxilin also regulates the placement of growing protein chains. Human alpha‑Taxilin is 546 amino acids (aa) in length. Its defining characteristic is a large coiled‑coil region between aa 186‑491. When an alternate start site at Met176 is used, a secreted cytokine is generated termed IL‑14 alpha. Over aa 1‑162, human alpha‑Taxilin shares 81% aa identity with mouse alpha‑Taxilin.
Alternate Names
alphaTaxilin, IL-14, TXLNA
Gene Symbol
TXLNA
UniProt
Additional alpha-Taxilin Products
Product Documents for Human alpha-Taxilin Antibody
Product Specific Notices for Human alpha-Taxilin Antibody
For research use only
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