U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human Aminopeptidase N/CD13 Monoclonal Antibody (Catalog # MAB38152, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Aminopeptidase N/CD13 in Human Liver.

Aminopeptidase N/CD13 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Aminopeptidase N/CD13 Monoclonal Antibody (Catalog # MAB38152) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to bile canaliculi. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Aminopeptidase N/CD13 in Human Kidney.

Aminopeptidase N/CD13 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Aminopeptidase N/CD13 Monoclonal Antibody (Catalog # MAB38152) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Aminopeptidase N/CD13 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: U937 Human cell line

Immunohistochemistry

0.5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver and human kidney

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aminopeptidase N/CD13

The human ANPEP gene encodes aminopeptidase N (APN), which is also known as microsomal aminopeptidase, alanyl aminopeptidase, aminopeptidase M, CD13, or membrane protein p161 (1‑3). The deduced amino acid sequence of human APN consists of a short cytoplasmic tail (residues 2 to 8), a transmembrane region (residue 9 to 32), a Ser/Thr rich region and a zinc metalloprotease domain (residues 69 to 966). The amino acid sequence of human APN is 78% and 77% identical to that of rat and mouse, respectively. Widely expressed in many cells, tissues and species, APN cleaves the N-terminal amino acids from bioactive peptides, leading to their inactivation or degradation. The roles of APN in many fields, such as neuroscience, hematopoeitic cells, immune system, angiogenesis, cancer and viral infection, have been reviewed (3).

References

Olsen, J. et al. (1988) FEBS Lett. 238:307.
Look, A.T. et al. (1989) J. Clin. Invest. 83:1299.
Turner, A.J. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 289, Academic Press, San Diego.

Alternate Names

Aminopeptidase M, ANPEP, APN, CD13, gp150, PEPN

Entrez Gene IDs

290 (Human); 16790 (Mouse); 397520 (Porcine); 102115663 (Cynomolgus Monkey)

Gene Symbol

ANPEP

UniProt

AAA51719

Additional Aminopeptidase N/CD13 Products

Product Documents for Human Aminopeptidase N/CD13 Antibody

Product Datasheets

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COA

SDS

Download SDS

Product Specific Notices for Human Aminopeptidase N/CD13 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Aminopeptidase N/CD13 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB38152

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Aminopeptidase N/CD13 Antibody

Detection of Aminopeptidase N/CD13 in U937 Human Cell Line by Flow Cytometry.