Human Annexin V Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3991
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Knockout Validated, Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 38701
Product Specifications
Immunogen
E. coli-derived recombinant human Annexin V
Met1-Asp320
Accession # P08758
Met1-Asp320
Accession # P08758
Specificity
Detects human Annexin V in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Annexin V Antibody
Detection of Human Annexin V by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human Annexin V Monoclonal Antibody (Catalog # MAB3991) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Annexin V at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Annexin V in Human Placenta.
Annexin V was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human Annexin V Monoclonal Antibody (Catalog # MAB3991) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Human Annexin V by Simple WesternTM.
Simple Western lane view shows lysates of human lung tissue, loaded at 0.2 mg/mL. A specific band was detected for Annexin V at approximately 39 kDa (as indicated) using 2.5 µg/mL of Mouse Anti-Human Annexin V Monoclonal Antibody (Catalog # MAB3991). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human Annexin V Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta
Sample: Immersion fixed paraffin-embedded sections of human placenta
Knockout Validated
Annexin V
is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in
Annexin V knockout HEK293T cell line.
Simple Western
2.5-50 µg/mL
Sample: Human lung tissue, A549 human lung carcinoma cell line exosomes, HT‑29 human colon adenocarcinoma cell line exosomes, and COLO 205 human colorectal adenocarcinoma cell line whole cell lysate (WCL)
Sample: Human lung tissue, A549 human lung carcinoma cell line exosomes, HT‑29 human colon adenocarcinoma cell line exosomes, and COLO 205 human colorectal adenocarcinoma cell line whole cell lysate (WCL)
Western Blot
0.25 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line
Reviewed Applications
Read 1 review rated 5 using MAB3991 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Annexin V
Additional Annexin V Products
Product Documents for Human Annexin V Antibody
Product Specific Notices for Human Annexin V Antibody
For research use only
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