Human Apolipoprotein A-I/ApoA1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3664
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Simple Western, Western Blot
Cited:
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Human Plasma ApoA1 and E. coli-derived recombinant human ApoA1
Asp25-Gln267
Accession # P02647
Asp25-Gln267
Accession # P02647
Specificity
Detects human ApoA1 in direct ELISAs and Western blots. In Western blots, less than 1% cross‑reactivity with recombinant human (rh) ApoA2, rhApoB100, and rhApoC2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Apolipoprotein A-I/ApoA1 Antibody
Detection of Human Apolipoprotein A‑I/ApoA1 by Western Blot.
Western blot shows human plasma. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Apolipoprotein A-I/ApoA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3664) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Apolipoprotein A-I/ApoA1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Apolipoprotein A‑I/ApoA1 by Simple WesternTM.
Simple Western lane view shows lysates of human serum, loaded at 1:5000. A specific band was detected for Apolipoprotein A-I/ApoA1 at approximately 32 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Apolipoprotein A-I/ApoA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3664) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human Apolipoprotein A-I/ApoA1 Antibody
Application
Recommended Usage
Simple Western
5 µg/mL
Sample: Human serum
Sample: Human serum
Western Blot
0.5 µg/mL
Sample: Human Plasma
Sample: Human Plasma
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Apolipoprotein A-I/ApoA1
Alternate Names
Alp-1, APOA1, Apolipoprotein AI, Brp-14, Ltw-1, Lvtw-1, Sep-1, Sep-2
Gene Symbol
APOA1
UniProt
Additional Apolipoprotein A-I/ApoA1 Products
Product Documents for Human Apolipoprotein A-I/ApoA1 Antibody
Product Specific Notices for Human Apolipoprotein A-I/ApoA1 Antibody
For research use only
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