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Human Apolipoprotein A-I/ApoA1 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF3664

R&D Systems, part of Bio-Techne
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BAF3664

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

E. coli-derived recombinant human ApoA1
Asp25-Gln267
Accession # P02647

Specificity

Detects human ApoA1 in Western blots. In this format, less than 1% cross-reactivity with recombinant human (rh) ApoA2, rhApoE, and rhApoE3 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Apolipoprotein A-I/ApoA1 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Apolipoprotein A-I/ApoA1
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Apolipoprotein A-I/ApoA1

ApoA1 is a major protein component of serum high-density lipoprotein (HDL) and is produced by the liver and small intestine. It is involved in reverse cholesterol transport from tissues to the liver. ApoA1 is synthesized as a 267 amino acid (aa) precursor from which a signal peptide and short propeptide are removed. Mature human ApoA1 shares 65% and 61% aa sequence identity with mouse and rat ApoA1, respectively.

Alternate Names

Alp-1, APOA1, Apolipoprotein AI, Brp-14, Ltw-1, Lvtw-1, Sep-1, Sep-2

Entrez Gene IDs

335 (Human); 11806 (Mouse); 25081 (Rat)

Gene Symbol

APOA1

UniProt

Additional Apolipoprotein A-I/ApoA1 Products

Product Documents for Human Apolipoprotein A-I/ApoA1 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Apolipoprotein A-I/ApoA1 Biotinylated Antibody

For research use only

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