I would like to buy antibodies APOA4 (apolipoprotein AIV) for the cows (to western blot). I know that You don't have the antibodies for this species. Could You propose the best atibodies which I could test (use) in my experiment?

All eight of our APOA4 antibodies were raised against the human protein, and all of them have currently only been tested against human samples. I ran an alignment for you and found that there is a 77% sequence homology between human and bovine APOA4, so it is possible that the antibodies raised against human APOA4 may recognise the bovine protein. Should you wish to try any of our antibodies with your samples, you may be interested in our Innovator's Reward Program, which allows you to try our primary antibodies in an untested species or application without the financial risk of failure. To participate you simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product.

Human Apolipoprotein A-IV/ApoA4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8125

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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 988829

View all Apolipoprotein A-IV/ApoA4 Antibodies »

Product Specifications

Immunogen

E. coli-derived human Apolipoprotein A-IV/ApoA4 protein
Glu21-Ser396
Accession # P06727

Specificity

Detects human Apolipoprotein A‑IV/ApoA4 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human Apolipoprotein A-IV/ApoA4 Antibody

Detection of Human Apolipoprotein A-IV/ApoA4 by Western Blot.

Western blot shows human plasma. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Apolipoprotein A-IV/ApoA4 Monoclonal Antibody (Catalog # MAB8125) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Apolipoprotein A-IV/ApoA4 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Apolipoprotein A-IV/ApoA4 in Human Intestine.

Apolipoprotein A-IV/ApoA4 was detected in immersion fixed paraffin-embedded sections of human intestine using Mouse Anti-Human Apolipoprotein A-IV/ApoA4 Monoclonal Antibody (Catalog # MAB8125) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human Apolipoprotein A-IV/ApoA4 by Simple Western^TM.

Simple Western lane view shows lysates of human plasma, loaded at 0.2 mg/mL. A specific band was detected for Apolipoprotein A-IV/ApoA4 at approximately 51 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Apolipoprotein A-IV/ApoA4 Monoclonal Antibody (Catalog # MAB8125) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Apolipoprotein A-IV/ApoA4 Antibody

Application

Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human intestine

Simple Western

20 µg/mL
Sample: Human Plasma

Western Blot

1 µg/mL
Sample: Human plasma

Reviewed Applications

Read 1 review rated 5 using MAB8125 in the following applications:

Immunohistochemistry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Apolipoprotein A-IV/ApoA4

Apolipoprotein A-IV (ApoA4) is a 45 kDa glycoprotein of the lipid transport system. Secreted in plasma, ApoA4 is a major component of high density lipoprotein (HDL) particles and chylomicrons, and is thought to act in intestinal lipid absorption. Levels of ApoA4 may influence HDL metabolism and modulate its effects on atherogenesis (1). ApoA4 synthesis in humans is mainly confined to the small intestine, while in mice and rats, production occurs in the liver as well (2). ApoA4 shares several structural characteristics with ApoA1 and other exchangeable apolipoproteins. The core domain of human ApoA4 contains thirteen 22-amino acid tandem repeats, and nine of which are predicted to be amphipathic alpha-helical repeats that are critical for lipid binding and self-association (3). The overall structure of a long rod‑like dimer consisting of two 4-helix bundles stacked end-to-end in opposing orientations (4). Human ApoA4 is synthesized as a 396 amino acid (aa) precursor, from which a 20 aa N-terminal signal peptide is removed. Mature human ApoA4 shares 61% and 62% aa sequence identity with mouse and rat ApoA4, respectively.

References

Duverger N. et al. (1991) Eur. J. Biochem. 201:373.
Maeda, N. et al. (1994) J. Biol. Chem. 269:23610.
Segrest, J. P. et al. (1994). Adv. Protein. Chem. 45:303.
Deng, X. et al. (2012) Structure 20:767.

Alternate Names

Apolipoprotein AIV

Entrez Gene IDs

337 (Human); 11808 (Mouse); 25080 (Rat)

Gene Symbol

APOA4

UniProt

P06727

Additional Apolipoprotein A-IV/ApoA4 Products

Product Documents for Human Apolipoprotein A-IV/ApoA4 Antibody

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Product Specific Notices for Human Apolipoprotein A-IV/ApoA4 Antibody

For research use only

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