Human ARNT/HIF-1 beta Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5630
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 775146
Product Summary for Human ARNT/HIF-1 beta Antibody
Immunogen
E. coli-derived recombinant human ARNT/HIF‑1 beta
Asp517-Ala691
Accession # P27540
Asp517-Ala691
Accession # P27540
Specificity
Detects human ARNT/HIF‑1 beta in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human ARNT/HIF-1 beta Antibody
Detection of Human ARNT/HIF‑1 beta by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ARNT/HIF-1 beta Monoclonal Antibody (Catalog # MAB5630) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for ARNT/HIF-1 beta at approximately 90-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
ARNT/HIF‑1 beta in MCF‑7 Human Cell Line.
ARNT/HIF-1 beta was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human ARNT/HIF-1 beta Monoclonal Antibody (Catalog # MAB5630) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human ARNT/HIF-1 beta Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed MCF‑7 human breast cancer cell line
Sample: Immersion fixed MCF‑7 human breast cancer cell line
Western Blot
2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line
Sample: HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ARNT/HIF-1 beta
Long Name
Aryl Hydrocarbon Receptor Nuclear Translocator
Alternate Names
HIF-1 beta, HIF1 beta, TANGO
Gene Symbol
ARNT
UniProt
Additional ARNT/HIF-1 beta Products
Product Documents for Human ARNT/HIF-1 beta Antibody
Product Specific Notices for Human ARNT/HIF-1 beta Antibody
For research use only
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