Human ARNT/HIF-1 beta Antibody

Name: Human ARNT/HIF-1 beta Antibody
Brand: R&D Systems
SKU: AF5630

R&D Systems, part of Bio-Techne | Catalog # AF5630

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunocytochemistry, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all ARNT/HIF-1 beta Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ARNT/HIF‑1 beta
Asp517-Ala691
Accession # P27540

Specificity

Detects human ARNT/HIF‑1 beta in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human ARNT/HIF-1 beta Antibody

Detection of Human ARNT/HIF‑1 beta by Western Blot.

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ARNT/HIF-1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5630) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ARNT/HIF-1 beta at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

ARNT/HIF‑1 beta in T47D Human Cell Line.

ARNT/HIF-1 beta was detected in immersion fixed T47D human breast cancer cell line using Goat Anti-Human ARNT/HIF-1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5630) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western Blot Shows Human ARNT/HIF‑1 beta Specificity by Using Knockout Cell Line.

Western blot shows lysates of A431 human epithelial carcinoma parental cell line and ARNT/HIF-1 beta knockout A431 cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ARNT/HIF-1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5630) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ARNT/HIF-1 beta at approximately 100 kDa (as indicated) in the parental A431 cell line, but is not detectable in knockout A431 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human ARNT/HIF-1 beta Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed T47D human breast cancer cell line

Knockout Validated

ARNT/HIF‑1 beta is specifically detected in A431 human epithelial carcinoma parental cell line but is not detectable in ARNT/HIF‑1 beta knockout A431 cell line.

Western Blot

1 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and K562 human chronic myelogenous leukemia cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ARNT/HIF-1 beta

ARNT (Aryl hydrocarbon nuclear translocator; also HIF-1 beta, Hypoxia-inducible factor 1-beta) is an 87-97 kDa member of the bHLH-PAS subclass of the bHLH family of transcription factors. It is widely expressed, and serves as part of both the NF kappaB and aryl hydrocarbon receptor (AHR) signaling nodes. For the AHR, the AHR is normally inactive and bound to hsp90 in the cytosol. Following ligand binding, the AHR complex enters the nucleus, dissociates from hsp90, dimerizes with ARNT, and then binds response elements of select genes. Human ARNT is 789 amino acids (aa) in length. It contains a DNA-binding region (aa 87‑143), two PAS domains (aa 161‑235 and 362‑458) and one PAC region (aa 424‑467). There are multiple potential splice forms. Two alternate start sites are found at Met10 and Met354, there is a deletion of aa 329‑755 and 77‑91, and an Ala substitution for aa 328‑789.

Long Name

Aryl Hydrocarbon Receptor Nuclear Translocator

Alternate Names

HIF-1 beta, HIF1 beta, TANGO

Entrez Gene IDs

405 (Human); 11863 (Mouse); 25242 (Rat)

Gene Symbol

ARNT

UniProt

P27540

Additional ARNT/HIF-1 beta Products

Product Documents for Human ARNT/HIF-1 beta Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human ARNT/HIF-1 beta Antibody

For research use only

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