Human Arylsulfatase B/ARSB Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4415
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 708401
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Arylsulfatase B/ARSB
Ser37-Met533
Accession # P15848
Ser37-Met533
Accession # P15848
Specificity
Detects human Arylsulfatase B/ARSB in direct ELISAs and Western blots. In direct ELISAs and Western blots, no
cross-reactivity with recombinant human (rh) ARSA, rhARSJ, or recombinant mouse
ARSG is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Arylsulfatase B/ARSB Antibody
Detection of Human Arylsulfatase B/ARSB by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Huh-7 human hepatoma cell line, and human skeletal muscle tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Arylsulfatase B/ARSB Monoclonal Antibody (Catalog # MAB4415) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Arylsulfatase B/ARSB at approximately 47 to 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Arylsulfatase B/ARSB in HepG2 Human Cell Line.
Arylsulfatase B/ARSB was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Arylsulfatase B/ARSB Monoclonal Antibody (Catalog # MAB4415) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Arylsulfatase B/ARSB Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line
Western Blot
1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, Huh‑7 human hepatoma cell line, and human skeletal muscle tissue
Sample: HepG2 human hepatocellular carcinoma cell line, Huh‑7 human hepatoma cell line, and human skeletal muscle tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB4415 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Arylsulfatase B/ARSB
Arylsulfatase B (ARSB) is a lysosomal enzyme of the sulfatase family that functions as an N-acetylgalactosamine-4-sulfatase (1). The enzyme is also active against chondroitin sulfate and dermatan sulfate (2). Deficiencies in ARSB activity are responsible for the lysosomal storage disease mucopolysaccharidosis Type VI, also known as Maroteaux-Lamy Syndrome (3).
References
- Peters, C. et al. (1990) J. Biol. Chem. 265:3374.
- O’Brien, J.S. et al. (1974) Biochem. Biophys. Res. Commun. 60:1170.
- Wicker, G. et al. (1991) J. Biol. Chem. 266:21386.
Alternate Names
ARSB, ASB, N-acetylgalactosamine-4-sulfatase
Gene Symbol
ARSB
UniProt
Additional Arylsulfatase B/ARSB Products
Product Documents for Human Arylsulfatase B/ARSB Antibody
Product Specific Notices for Human Arylsulfatase B/ARSB Antibody
For research use only
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