Human ASK1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3575
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human ASK1
Lys1011-Asp1196
Accession # Q99683
Lys1011-Asp1196
Accession # Q99683
Specificity
Detects human ASK1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human ASK1 Antibody
Detection of Human ASK1 by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ASK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3575) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ASK1 at approximately 154 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human ASK1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells
Sample: Immersion fixed human peripheral blood mononuclear cells
Western Blot
1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line
Sample: Raji human Burkitt's lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using AF3575 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ASK1
Apoptosis signal-regulating kinase 1 (ASK1) is a MAP kinase kinase kinase also known as MEKK5 and MAP3K5. ASK1 is induced by inflammatory cytokines, UV light, and other stress-related stimuli to phosphorylate MAP kinase kinases that in turn activate the p38 and JNK families of MAP kinases. Mice lacking ASK1 are resistant to stress-induced p38 and JNK activation and cell death.
Long Name
Apoptosis Signal-regulated Kinase 1
Alternate Names
MAP3K5, MEKK5
Gene Symbol
MAP3K5
UniProt
Additional ASK1 Products
Product Documents for Human ASK1 Antibody
Product Specific Notices for Human ASK1 Antibody
For research use only
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