Human ATRIP Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1579
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Simple Western, Western Blot
Cited:
Neutralization
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human ATRIP
Glu461-Gly791
Accession # Q8WXE1
Glu461-Gly791
Accession # Q8WXE1
Specificity
Detects endogenous human ATRIP in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human ATRIP Antibody
Detection of Human ATRIP by Western Blot.
Western blot shows lysates of 293T human embryonic kidney cell line, MCF-7 human breast cancer cell line and PC-3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Human ATRIP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1579) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ATRIP at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human and Mouse ATRIP by Simple WesternTM.
Simple Western lane view shows lysates of MCF-7 human breast cancer cell line and LL/2 mouse Lewis lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ATRIP at approximately 100 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human ATRIP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1579) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human ATRIP Antibody
Application
Recommended Usage
Simple Western
10 µg/mL
Sample: MCF‑7 human breast cancer cell line and LL/2 mouse Lewis lung carcinoma cell line
Sample: MCF‑7 human breast cancer cell line and LL/2 mouse Lewis lung carcinoma cell line
Western Blot
1 µg/mL
Sample: 293T human embryonic kidney cell line, MCF-7 human breast cancer cell line and PC-3 human prostate cancer cell line
Sample: 293T human embryonic kidney cell line, MCF-7 human breast cancer cell line and PC-3 human prostate cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ATRIP
ATRIP (ATR interacting protein) is an 85‑90 kDa member of the ATRIP family of proteins. It is ubiquitously expressed, and recruits ATR to sites of DNA damage and replication stress. Human ATRIP is 791 amino acids (aa) in length. It contains a PRA-ssDNA binding region (aa 1‑107), a coiled-coil domain (aa 108‑217) and an ATR‑recruitment region (aa 641‑726). The coiled-coil region mediates ATRIP oligomerization, and multimerization with ATR, creating complexes of 1000 kDa. ATRIP undergoes phosphorylation at Ser224, Ser239 and Ser518 influencing its role in the DNA damage response. There is an alternate start site at Met94, plus a deletion of aa 658‑684 in a second isoform, and a deletion of aa 661‑687 in a third isoform. Over aa 461‑791, human ATRIP is 76% aa identical to mouse ATRIP.
Long Name
Ataxia Telangiectasia and Rad 3-related Interacting Protein
Alternate Names
TREX1
Gene Symbol
ATRIP
UniProt
Additional ATRIP Products
Product Documents for Human ATRIP Antibody
Product Specific Notices for Human ATRIP Antibody
For research use only
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