Human Axin-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6078
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 354214
Product Specifications
Immunogen
E. coli-derived recombinant human Axin-2
Tyr550-Gln760
Accession # Q9Y2T1
Tyr550-Gln760
Accession # Q9Y2T1
Specificity
Detects human Axin-2 in direct ELISAs. In this format, no cross-reactivity with recombinant human Axin-1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Axin-2 Antibody
Axin‑2 in SW480 Human Cell Line.
Axin-2 was detected in immersion fixed SW480 human colorectal adenocarcinoma cell line using Mouse Anti-Human Axin-2 Monoclonal Antibody (Catalog # MAB6078) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Axin-2 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion-fixed SW480 human colorectal adenocarcinoma cell line
Sample: Immersion-fixed SW480 human colorectal adenocarcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Axin-2
Axin-2, also known as Conductin, is a 95 kDa cytoplasmic protein that inhibits Wnt signaling and promotes epithelial-mesenchymal transition. Axin 2 binds to beta-catenin, glycogen synthase kinase-3 (GSK-3 beta), and the tumor suppressor APC. Loss of Axin-2 function is linked to carcinogenesis as well as abnormal bone and tooth development. Within aa 550-760, human Axin-2 shares 82% aa sequence identity with mouse and rat Axin-2.
Long Name
Axis Inhibitor 2
Alternate Names
AXIL, Axin2, Conductin, MGC10366, MGC126582
Entrez Gene IDs
8313 (Human)
Gene Symbol
AXIN2
UniProt
Additional Axin-2 Products
Product Documents for Human Axin-2 Antibody
Product Specific Notices for Human Axin-2 Antibody
For research use only
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