Detects human B7‑1/CD80 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) B7-2, recombinant mouse (rm) B7-1, rmB7-2, rhB7-H1, rhB7-H2, rhB7-H3 or rmPD-L2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human B7-1/CD80 Fluorescein-conjugated Antibody

Detection of B7-1/CD80 in Human PBMC by Flow Cytometry.

Human PBMC were treated with 1μg/mL LPS overnight then stained with (A) Mouse Anti-Human B7-1/CD80 CFS-conjugated Monoclonal Antibody (Catalog # FAB140F) or (B) Mouse IgG1 Isotype Control (IC002F) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (FAB3832P). Staining was performed using our Staining Membrane-associated Proteins protocol.

Detection of B7-1/CD80 in Raji Human Cell Line by Flow Cytometry.

Raji human Burkitt's lymphoma cell line was stained with Mouse Anti-Human B7-1/CD80 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB140F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human B7-1/CD80 Fluorescein-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human PBMC treated with LPS and Raji human Burkitt's lymphoma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: B7-1/CD80

B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down‑regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up-regulated through Interferon gamma. B7-1 and B7-2 are both members of the Immunoglobulin superfamily. Human B7-1 is a 288 amino acid (aa) protein containing a 34 aa signal peptide, a 208 aa extracellular domain, a 21 aa transmembrane domain, and a 25 aa cytoplasmic domain. Human B7-1 and B7-2 share 26% aa sequence identity. Human and mouse B7-1 share 44% aa sequence identity. However, it has been observed that both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form the B7-1/B7-2/CD28/CTLA-4 critical binding sites.

References

Azuma, M. et al. (1993) Nature 366:76.
Freeman, G.J. et al. (1993) Science 262:909.
Freeman, G. et al. (1991) J. Exp. Med. 174:625.
Selvakumar, A. et al. (1993) Immunogenetics 38:292.
Chen, C. et al. (1994) J. Immunol. 152:4929.
Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.

Alternate Names

B71, CD80

Entrez Gene IDs

941 (Human); 12519 (Mouse); 25408 (Rat); 102140078 (Cynomolgus Monkey)

Gene Symbol

CD80

UniProt

P33681

Additional B7-1/CD80 Products

Product Documents for Human B7-1/CD80 Fluorescein-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human B7-1/CD80 Fluorescein-conjugated Antibody

For research use only

Citations
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Spectra Viewer
Flow Cytometry

Human B7-1/CD80 Fluorescein-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB140F

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications