Human Bax Minus C-Terminus Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB846
Key Product Details
Validated by
Knockout/Knockdown, Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Knockout Validated, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 127606
Product Specifications
Immunogen
E. coli-derived recombinant human Bax aa 1-171
Accession # Q07812
Accession # Q07812
Specificity
Detects full-length human Bax in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Bax Minus C-Terminus Antibody
Detection of Human Bax Minus C-Terminus by Western Blot.
Western blot shows lysate of THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human Bax Minus C-Terminus Monoclonal Antibody (Catalog # MAB846) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Bax Minus C-Terminus at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Bax in A549 Human Cell Line.
Bax was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human Bax Minus C-Terminus Monoclonal Antibody (Catalog # MAB846) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Western Blot Shows Human Bax Specificity by Using Knockout Cell Line.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Bax knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Bax Minus C-Terminus Monoclonal Antibody (Catalog # MAB846) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Bax at approximately 20 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Bax Minus C-Terminus Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed A549 human lung carcinoma cell line
Sample: Immersion fixed A549 human lung carcinoma cell line
Knockout Validated
Bax is
specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Bax
knockout HeLa cell line.
Western Blot
1 µg/mL
Sample: THP-1 human acute monocytic leukemia cell line
Sample: THP-1 human acute monocytic leukemia cell line
Reviewed Applications
Read 1 review rated 4 using MAB846 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Bax
Long Name
Bcl Associated X Protein
Alternate Names
apoptosis regulator BAX, BCL2-associated X protein, Bcl2-L-4, BCL2L4bcl2-L-4, Bcl-2-like protein 4
Gene Symbol
BAX
UniProt
Additional Bax Products
Product Documents for Human Bax Minus C-Terminus Antibody
Product Specific Notices for Human Bax Minus C-Terminus Antibody
For research use only
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