Human beta-TrCP1/BTRC Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7675

R&D Systems, part of Bio-Techne
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MAB7675
MAB7675-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 763524

View all beta-TrCP1/BTRC Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human beta-TrCP1 isoform 2
Met1-Leu120
Accession # Q9Y297-2

Specificity

Detects human BetaTrCP1 isoform 2 in direct ELISAs and Western blots. Detects mouse BetaTrCP1 in Western Blots. Detection of mouse BetaTrCP1 in Immuncytochemistry has not been tested.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human beta-TrCP1/BTRC Antibody

Detection of Human and Mouse beta-TrCP1/BTRC antibody by Western Blot.

Detection of Human and Mouse beta-TrCP1/BTRC by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human beta-TrCP1/BTRC Monoclonal Antibody (Catalog # MAB7675) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for beta-TrCP1/BTRC at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
beta-TrCP1/BTRC antibody in A172 Human Cell Line by Immunocytochemistry (ICC).

beta‑TrCP1/BTRC in A172 Human Cell Line.

beta-TrCP1/BTRC was detected in immersion fixed A172 human glioblastoma cell line using Mouse Anti-Human beta-TrCP1/BTRC Monoclonal Antibody (Catalog # MAB7675) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human beta-TrCP1/BTRC antibody by Simple WesternTM.

Detection of Human beta-TrCP1/BTRC by Simple WesternTM.

Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.5 mg/mL. A specific band was detected for beta-TrCP1/BTRC at approximately 84 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human beta-TrCP1/BTRC Monoclonal Antibody (Catalog # MAB7675). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human beta-TrCP1/BTRC Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed A172 human glioblastoma cell line

Simple Western

10 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line

Western Blot

1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line and C2C12 mouse myoblast cell line

Reviewed Applications

Read 1 review rated 5 using MAB7675 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-TrCP1/BTRC

beta-TrCP1, also called BTRC ( beta-transducin repeat containing E3 ubiquitin ligase), FBW1A (F-box/WD repeat containing 1A) and FBXW1A, is an approximately 63-70 kDa protein that associates with Skp1, Cullin, and RBX1 to form an SCF ubiquitin ligase complex. These complexes regulate the degradation of a wide variety of proteins including transcription factors, signal transduction molecules, and cytokine receptors. They play important roles in regulation of the inflammatory response, cell cycle, apoptosis, tumorigenesis, and the response to DNA damage. beta-TrCP1 can form homodimers and heterodimers with beta-TrCP2, which associate into SCF complexes with distinct substrate preferences. Human beta-TrCP1 contains a dimerization domain (aa 128-177), an F-box domain (aa 181-228), and seven tandem WD repeats (aa 301‑590). The WD repeats assemble into a beta propeller structure that binds to phosphorylated destruction motifs in the substrate. Alternate splicing generates a short isoform with a 36 aa deletion near the N-terminus. Within aa 1-120 of the 63 kDa short isoform, human beta-TrCP1 shares 98% and 91% aa sequence identity with mouse and rat beta-TrCP1, respectively.

Long Name

Beta-Transducin Repeat Containing E3 Ubiquitin Protein Ligase

Alternate Names

betaTrCP1, BTRC, BTRCP, E3RSIkappaB, FBW1A, FBXW1A, FWD1

Entrez Gene IDs

8945 (Human); 12234 (Mouse); 361765 (Rat)

Gene Symbol

BTRC

UniProt

Q9Y297-2

Additional beta-TrCP1/BTRC Products

Product Documents for Human beta-TrCP1/BTRC Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human beta-TrCP1/BTRC Antibody

For research use only

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