Human BMP-2/BMP-7 Heterodimer Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3229
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 369505
Product Specifications
Immunogen
E. coli-derived recombinant human BMP‑2/BMP‑7 Heterodimer
Ala284-Arg396 (BMP-2), Ser293-His431 (BMP-7)
Accession # NP_001191, NP_001710
Ala284-Arg396 (BMP-2), Ser293-His431 (BMP-7)
Accession # NP_001191, NP_001710
Specificity
Detects human BMP‑2/BMP‑7 Heterodimer in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human (rh) BMP-2 is observed, and no cross-reactivity with rhBMP-4/7, -4, -5, -6, -7, or -8 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human BMP-2/BMP-7 Heterodimer Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human BMP-2/BMP-7 Heterodimer (Catalog # 3229-BM) under non-reducing conditions only
Sample: Recombinant Human BMP-2/BMP-7 Heterodimer (Catalog # 3229-BM) under non-reducing conditions only
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMP-2/BMP-7 Heterodimer
Bone Morphgenetic Protein 7 (BMP-7), also known as osteogenic protein 1 (OP-1), and BMP-2 are members of the BMP subgroup of the TGF-beta superfamily and signal through heterodimeric complexes composed of type I and type II BMP receptors. After proteolytic removal of their propeptides, BMP-2 and BMP-7 can associate into 38 kDa disulfide-linked heterodimers. The heterodimers are significantly more potent than either homodimer in osteoblast differentiation and bone formation assays. Human and mouse BMP-2 and BMP-7 share 100% and 98% aa sequence identity, respectively.
Long Name
Bone Morphogenetic Protein 2/7
Alternate Names
BDA2;BMP2A;Bone morphogenetic protein 2;SSFSC;SSFSC1
Entrez Gene IDs
650 (Human)
Gene Symbol
BMP2
UniProt
Additional BMP-2/BMP-7 Heterodimer Products
Product Documents for Human BMP-2/BMP-7 Heterodimer Antibody
Product Specific Notices for Human BMP-2/BMP-7 Heterodimer Antibody
For research use only
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