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Human BMP-7 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAM354

R&D Systems, part of Bio-Techne
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BAM354

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

ELISA Detection (Matched Antibody Pair)

Cited:

ELISA Detection

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 Clone # 164324

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human BMP-7
Arg292-His431
Accession # P18075

Specificity

Detects human BMP-7 in ELISAs. In ELISAs, this antibody shows less than 0.125% cross-reactivity with recombinant human (rh) BMP‑2, rhBMP‑3, rhBMP‑4, rhBMP‑5, rhBMP‑6, rhBMP‑8, rhTGF‑ beta1, rhTGF‑ beta2, or rhTGF‑ beta3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human BMP-7 Biotinylated Antibody

Human BMP-7 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.5-2.0 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human BMP-7 Antibody (Catalog # MAB3542)
  • Standard: Recombinant Human BMP-7 Protein (Catalog # 354-BP)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-7

BMP-7, also known as Osteogenic Protein 1 (OP-1), is one of at least 15 structurally and functionally related BMPs, which are members of the TGF-beta superfamily. BMPs regulate cartilage and bone formation, embryogenesis and morphogenesis of various tissues and organs, and growth, differentiation, chemotaxis, and apoptosis of various cell types. Biologically active BMP-7 is a disulfide-linked homodimer. Cellular responses to BMP-7 have been shown to be mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors.

Long Name

Bone Morphogenetic Protein 7

Alternate Names

BMP7, OP-1

Entrez Gene IDs

655 (Human); 12162 (Mouse); 85272 (Rat)

Gene Symbol

BMP7

UniProt

Additional BMP-7 Products

Product Documents for Human BMP-7 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human BMP-7 Biotinylated Antibody

For research use only

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