Human Bora Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6856

R&D Systems, part of Bio-Techne
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MAB6856
MAB6856-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 694730

View all Bora Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Bora
Gly2-Ala180
Accession # Q6PGQ7

Specificity

Detects human Bora in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Bora Antibody

Bora antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC).

Bora in MCF‑7 Human Cell Line.

Bora was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human Bora Monoclonal Antibody (Catalog # MAB6856) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Bora Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MCF‑7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Bora

Bora (Aurora borealis; also C13orf34) is a 61 kDa member of the Bora family of proteins.  It is ubiquitously expressed, and plays a key role in cell cycle progression.  Plk1 (polo-like kinase-1) is a phosphorylase that is important to the cell during the G2/M transition and mitosis.  Its activity is initially regulated by Aurora-A, which phosphorylates and activates Plk1 on Thr210.  Bora, Aurora-A and Plk1 all appear to form a complex during G2.  Bora predisposes Plk1 to the actions of Aurora-A.  Once activated by Aurora-A, Plk1 drives the mitotic mechanism, which includes a third-party phosphorylation of Bora.  This initiates BORA dissociation from Aurora-A with subsequent ubiquitination and degradation.  Human Bora is 559 amino acids (aa) in length. It contains a Ser-rich region (aa 188-278) and at least eight utilized Ser phosphorylation sites.  Phosphorylation may increase the SDS-PAGE MW of Bora to 75-85 kDa.  There is one potential alternative start site that lies 60 aa upstream of the standard start site, and a second splice variant the shows a 17 aa substitution for aa 1-87.  Over aa 2-180, human Bora shares 84% aa identity with mouse Bora.

Long Name

Protein Aurora Borealis

Alternate Names

Aurora Borealis, C13orf34

Entrez Gene IDs

79866 (Human); 777441 (Mouse); 306102 (Rat)

Gene Symbol

BORA

UniProt

Q6PGQ7 (Human)

Additional Bora Products

Product Documents for Human Bora Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Bora Antibody

For research use only

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