HEK293 human embryonic kidney cell line transfected with (A) human Bradykinin RB2/BDKRB2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Bradykinin RB2/BDKRB2 Monoclonal Antibody (Catalog # MAB47251) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Bradykinin RB2/BDKRB2 in Human Stomach.

Bradykinin RB2/BDKRB2 was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human Bradykinin RB2/BDKRB2 Monoclonal Antibody (Catalog # MAB47251) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Bradykinin RB2/BDKRB2 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human Bradykinin RB2/BDKRB2 and eGFP

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human stomach

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Bradykinin RB2/BDKRB2

Bradykinin RB2 (BDKRB2) is a receptor for bradykinin. The 9 aa bradykinin peptide elicits many responses including vasodilation, edema, smooth muscle spasm and pain fiber stimulation. BDKRB2 expression is widespread in normal smooth muscle tissue and neurons. BDKRB2 associates with G proteins that stimulate a phosphatidylinositol-calcium second messenger system. BDKRB2 forms a complex with PECAM1 and GNAQ and interacts with PECAM1. Aging cardiac endothelial cells gradually lose their capacity to express BDKRB2. This loss appears to be parallel with a loss of the angiogenic potential of the aging cells.

Long Name

Bradykinin Receptor B2

Alternate Names

B2R, BDKRB2, BKR2, BradykininRB2, BRB2

Entrez Gene IDs

624 (Human); 12062 (Mouse); 25245 (Rat)

Gene Symbol

BDKRB2

UniProt

P30411

Additional Bradykinin RB2/BDKRB2 Products

Product Documents for Human Bradykinin RB2/BDKRB2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Bradykinin RB2/BDKRB2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Bradykinin RB2/BDKRB2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB47251

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Bradykinin RB2/BDKRB2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Bradykinin RB2/BDKRB2 Antibody

Detection of Bradykinin RB2/BDKRB2 in HEK293 Human Cell Line Transfected with Human Bradykinin RB2/BDKRB2 and eGFP by Flow Cytometry.