Human BTNL8 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11364

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
MAB11364-100
MAB11364-SP
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1062005

View all BTNL8 Antibodies »

Product Specifications

Immunogen

Synthetic peptide Fc-cleaved human BTNL8 

Specificity

Detects human BTNL8 in direct ELISA

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human BTNL8 Antibody

Detection of BTNL8 in Caco-2 cells (positive) and HepG2 cells (negative).

BTNL8 was detected in immersion fixed Caco-2 human colorectal adenocarcinoma cells (positive) and absent in HepG2 human hepatocellular carcinoma cells (negative) using Mouse Anti-Human BTNL8 Monoclonal Antibody (Catalog # MAB11364) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human BTNL8 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Caco-2 human colorectal adenocarcinoma cells (positive) and HepG2 human hepatocellular carcinoma cells (negative)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BTNL8

Butyrophilin-like 8 (BTNL8) is a member of the BTN/MOG Ig-superfamily and functions as a negative regulator of immune cell activation (1). Human BTNL8 is a 500 amino acid (aa) type I transmembrane glycoprotein that contains a signal peptide followed by an extracellular domain (ECD), a transmembrane region and a short cytoplasmic domain (2). The ECD of human BTNL8 shares 88% sequence identity with the ECD of mouse BTNL8. BTNL8 has two alternatively spliced forms: B7-like and BTN-like. Both isoforms of BTNL8 are expressed in a range of human tissues (3). The complete immunological function of BTNL molecules is only beginning to emerge. BTNL8 has been shown to be important in initiation of primary immune responses, suggesting a role in priming of naïve T lymphocytes (3). Down-regulation of BNTL8 mRNA levels has been associated with ulcerative colitis and colon cancer (4). BTNL8 are expressed in colon, lung, testis and neutrophils, and its expression is significantly decreased in ulcerative colitis, colonic tumors as compared to unaffected tissue (4). Soluble BTNL8-Fc fusion protein binds to resting, but not activated T cells. In vitro, BTNL8 co-stimulates T cell proliferation and cytokine production. In vivo injections of BTNL8-Fc significantly increases production of Ag-specific IgG during the primary but not the secondary immune response (3).

References

  1. Arnett, H.A. et al. (2007) J. Immunol. 178:1523.
  2. Arnett, H.A. et al. (2009) Cytokine 46:370.
  3. Chapoval, A.I. et al. (2013) Mol Immunol. 56:819.
  4. Lebrero-Fernández C. et al. (2016) Immun Inflamm Dis. 4:191.

Long Name

Butyrophilin-like 8

Alternate Names

BTN9.2

Entrez Gene IDs

79908 (Human); 406160 (Rat)

Gene Symbol

BTNL8

Additional BTNL8 Products

Product Documents for Human BTNL8 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human BTNL8 Antibody

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.