Human c-Fos Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7254
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human c-Fos
Met1-Leu132
Accession # P01100
Met1-Leu132
Accession # P01100
Specificity
Detects human c-Fos in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human Fos-B is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human c-Fos Antibody
Detection of Human c-Fos by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for 4 hours. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human c-Fos Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7254) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for c-Fos at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
c‑Fos in HeLa Human Cell Line.
c-Fos was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human c-Fos Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7254) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human c-Fos Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Western Blot
0.2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line treated with PMA
Sample: HeLa human cervical epithelial carcinoma cell line treated with PMA
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: c-Fos
Long Name
FBJ/Finkel–Biskis–Jinkins Murine Osteosarcoma Viral Oncogene Homolog
Alternate Names
cFos, FOS, G0S7
Gene Symbol
FOS
UniProt
Additional c-Fos Products
Product Documents for Human c-Fos Antibody
Product Specific Notices for Human c-Fos Antibody
For research use only
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