Human Cadherin-17 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1032
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 141713
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cadherin-17
Gln23-Met787
Accession # CAA58231
Gln23-Met787
Accession # CAA58231
Specificity
Detects human Cadherin-17 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) Cadherin‑8, rhE‑Cadherin, rhP‑Cadherin, or rhVE‑Cadherin.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Cadherin-17 Antibody
Detection of Human Cadherin-17 by Western Blot.
Western blot shows lysates of human colon tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Cadherin-17 Monoclonal Antibody (Catalog # MAB1032) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Cadherin-17 at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cadherin‑17 in Human Ascending Colon.
Cadherin-17 was detected in immersion fixed paraffin-embedded sections of human ascending colon using Mouse Anti-Human Cadherin-17 Monoclonal Antibody (Catalog # MAB1032) at 0.5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue).
Cadherin‑17 in Human Transverse Colon.
Cadherin-17 was detected in immersion fixed paraffin-embedded sections of human transverse colon using Mouse Anti-Human Cadherin-17 Monoclonal Antibody (Catalog # MAB1032) at 0.5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue).Applications for Human Cadherin-17 Antibody
Application
Recommended Usage
Immunohistochemistry
0.5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human ascending and transverse colon
Sample: Immersion fixed paraffin-embedded sections of human ascending and transverse colon
Western Blot
1 µg/mL
Sample: Human colon tissue
Sample: Human colon tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cadherin-17
Alternate Names
Cadherin17, CDH17
Gene Symbol
CDH17
UniProt
Additional Cadherin-17 Products
Product Documents for Human Cadherin-17 Antibody
Product Specific Notices for Human Cadherin-17 Antibody
For research use only
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