Human Caldesmon/CALD1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7569
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 767001
Product Specifications
Immunogen
E. coli-derived recombinant human Caldesmon/CALD1
Lys696-Val793
Accession # Q05682
Lys696-Val793
Accession # Q05682
Specificity
Detects human Caldesmon/CALD1 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Caldesmon/CALD1 Antibody
Detection of Human Caldesmon/CALD1 by Western Blot.
Western blot shows lysates of WI-38 human lung fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Caldesmon/CALD1 Monoclonal Antibody (Catalog # MAB7569) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Caldesmon/CALD1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Caldesmon/CALD1 in WI‑38 Human Cell Line.
Caldesmon/CALD1 was detected in immersion fixed WI-38 human lung fibroblast cell line using Mouse Anti-Human Caldesmon/CALD1 Monoclonal Antibody (Catalog # MAB7569) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counter-stained with DAPI (blue). Specific staining was localized to cytoskeleton. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Caldesmon/CALD1 in Endometrial Carcinoma.
Caldesmon/CALD1 was detected in immersion fixed paraffin-embedded sections of endometrial carcinoma using Mouse Anti-Human Caldesmon/CALD1 Monoclonal Antibody (Catalog # MAB7569) at 1 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Caldesmon/CALD1 Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed WI‑38 human lung fibroblast cell line
Sample: Immersion fixed WI‑38 human lung fibroblast cell line
Immunohistochemistry
3-25 µg/mL
Sample:
Sample:
Immersion fixed paraffin-embedded sections of endometrial carcinoma and melanoma
Western Blot
2 µg/mL
Sample: WI‑38 human lung fibroblast cell line
Sample: WI‑38 human lung fibroblast cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Caldesmon/CALD1
Additional Caldesmon/CALD1 Products
Product Documents for Human Caldesmon/CALD1 Antibody
Product Specific Notices for Human Caldesmon/CALD1 Antibody
For research use only
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