Human Carboxypeptidase A2/CPA2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2896
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
NS0-derived rhCPA2 (aa 17 - 417)
aa 17-419
aa 17-419
Specificity
Detects human CPA2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with recombinant human (rh) CPA1 is observed and less than 1% cross-reactivity with rhCPB1 and recombinant mouse CPA4 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human Carboxypeptidase A2/CPA2 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Carboxypeptidase A2/CPA2 (Catalog # 2896-ZN), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Carboxypeptidase A2/CPA2 (Catalog # 2896-ZN), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Human Carboxypeptidase A2 (Catalog # 2896-ZN)
Sample: Recombinant Human Carboxypeptidase A2 (Catalog # 2896-ZN)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carboxypeptidase A2/CPA2
Carboxypeptidase A2 encoded by the CPA2 gene cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group (1). It prefers the C-terminal residues with aromatic side chains including Phe, Tyr, and Trp. The deduced amino acid sequence of human CPA2 consists of a signal peptide (residues 1 to 16), a pro region (residues 17 to 112), and a mature chain (residues 113 to 417). The purified rhCPA2 corresponds to the pro form, which can be activated and assayed.
References
- Auld, D.S. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 821, Academic Press, San Diego.
Alternate Names
CPA2
Entrez Gene IDs
1358 (Human)
Gene Symbol
CPA2
Additional Carboxypeptidase A2/CPA2 Products
Product Documents for Human Carboxypeptidase A2/CPA2 Antibody
Product Specific Notices for Human Carboxypeptidase A2/CPA2 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...