Human Carboxypeptidase A2/CPA2 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF2896
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carboxypeptidase A2/CPA2
Tyr17-His417
Accession # P48052
Tyr17-His417
Accession # P48052
Specificity
Detects human Carboxypeptidase A2/CPA2 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) CPA1, rhCPB1, and recombinant mouse CPA4 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human Carboxypeptidase A2/CPA2 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human Carboxypeptidase A2 (Catalog # 2896-ZN)
Sample: Recombinant Human Carboxypeptidase A2 (Catalog # 2896-ZN)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carboxypeptidase A2/CPA2
Carboxypeptidase A2 encoded by the CPA2 gene cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group (1). It prefers the C-terminal residues with aromatic side chains including Phe, Tyr, and Trp. The deduced amino acid sequence of human CPA2 consists of a signal peptide (residues 1 to 16), a pro region (residues 17 to 112), and a mature chain (residues 113 to 417). The purified rhCPA2 corresponds to the pro form, which can be activated and assayed.
References
- Auld, D.S. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 821, Academic Press, San Diego.
Alternate Names
CPA2
Entrez Gene IDs
1358 (Human)
Gene Symbol
CPA2
UniProt
Additional Carboxypeptidase A2/CPA2 Products
Product Documents for Human Carboxypeptidase A2/CPA2 Biotinylated Antibody
Product Specific Notices for Human Carboxypeptidase A2/CPA2 Biotinylated Antibody
For research use only
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