Human Carnosine Dipeptidase 1/CNDP1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB24891
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 352213
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carnosine Dipeptidase 1/CNDP1
Pro28-His507
Accession # Q96KN2
Pro28-His507
Accession # Q96KN2
Specificity
Detects human Carnosine Dipeptidase 1/CNDP1 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Applications for Human Carnosine Dipeptidase 1/CNDP1 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Carnosine Dipeptidase 1/CNDP1 (Catalog # 2489-ZN), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Carnosine Dipeptidase 1/CNDP1 (Catalog # 2489-ZN), see our available Western blot detection antibodies
Neutralization
Measured by its ability to neutralize Recombinant Human Carnosine Dipeptidase 1/CNDP1 (0.5 µg/mL, Catalog # 2489-ZN) cleavage of the fluorogenic peptide substrate L-Carnosine (0.5 mM). The Neutralization Dose (ND50) is typically 0.6 µg/mL.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carnosine Dipeptidase 1/CNDP1
The human CNDP1 gene encodes carnosine dipeptidase 1, a member of the M20 family of metalloproteases (1, 2). Also known as X-His dipeptidase, glutamate carboxypeptidase-like protein 2 (CPGL-2) or carnosinase 1 (CN1), CNDP1 is a secreted dipeptidase with a narrow specificity for Xaa-His dipeptides including those with Xaa = beta-Ala (carnosine) and Xaa = gamma-aminobutyric acid (homocarnosine), two naturally occurring dipeptides with potential neuroprotective and neurotransmitter fucntions in the brain. In comparison, a closely related protein known as CNDP2, CPGL or CN2, is a cytosolic nonspecific dipeptidase. CNDP1 consists of a signal peptide (residues 1 to 26) and the mature chain (residues 27 to 507). The amino acid sequence of human CNDP1 is 97%, 85%, 82% and 67% identical to that of chimpanzee, canine, mouse/rat and chicken.
References
- Teufel, M. et al. (2004) J. Biol. Chem. 278:6521.
- Bauer, K. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 1022, Academic Press, San Diego.
Alternate Names
Carnosinase 1, CNDP1, CPGL2
Entrez Gene IDs
84735 (Human)
Gene Symbol
CNDP1
UniProt
Additional Carnosine Dipeptidase 1/CNDP1 Products
Product Documents for Human Carnosine Dipeptidase 1/CNDP1 Antibody
Product Specific Notices for Human Carnosine Dipeptidase 1/CNDP1 Antibody
For research use only
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