Human Caspase-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB62151
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Applications
ELISA, Knockout Validated, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 1021430
Product Summary for Human Caspase-1 Antibody
Immunogen
E. coli-derived recombinant human Caspase-1
Asn120-Asp297
Accession # P29466
Asn120-Asp297
Accession # P29466
Specificity
Detects human Caspase-1 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Caspase-1 Antibody
Detection of Human Caspase‑1 by Western Blot.
Western blot shows lysates of THP-1 human acute monocytic leukemia cell line and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Caspase-1 Monoclonal Antibody (Catalog # MAB62151) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Caspase-1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Caspase‑1 by Simple WesternTM.
Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Caspase‑1 at approximately 52 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Caspase‑1 Monoclonal Antibody (Catalog # MAB62151) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Human Caspase-1 ELISA Standard Curve.
Recombinant Human Caspase-1 protein was serially diluted 2-fold and captured by Mouse Anti-Human Caspase-1 Monoclonal Antibody (Catalog # MAB62151) coated on a Clear Polystyrene (DY990) Microplate. Mouse Anti-Human Caspase-1 Monoclonal Antibody (MAB62153) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).Applications for Human Caspase-1 Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human Caspase‑1 Monoclonal Antibody (Catalog # MAB62153).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Caspase-1/ICE Quantikine ELISA Kit (Catalog # DCA100) for a complete optimized ELISA.
Knockout Validated
Caspase‑1
is specifically detected in A431 human epithelial carcinoma parental cell line but is not detectable in
Caspase‑1 knockout A431 cell line.
Simple Western
20 µg/mL
Sample: A431 human epithelial carcinoma cell line
Sample: A431 human epithelial carcinoma cell line
Western Blot
2 µg/mL
Sample: THP‑1 human acute monocytic leukemia cell line and A431 human epithelial carcinoma cell line
Sample: THP‑1 human acute monocytic leukemia cell line and A431 human epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Caspase-1
Caspase-1, also known as IL-1 beta-Converting Enzyme (ICE), is an aspartic protease that plays a key role in the inflammatory response and apoptosis. Caspase-1 precursor (about 50kDa) can be cleaved and the active enzyme consists of a complex of two 20 kDa (aa 120-297) and two 10 kDa (aa 317-404) subunits which associate following cleavage of inactive precursors. Caspase-1 is required for proteolytic cleavage of the IL-1 beta precursor to form the active proinflammatory cytokine. Alternate splicing generates several additional Caspase-1 isoforms with deletions in the propeptide regions or also in the mature subunits. Within the large subunit, human Caspase 1 shares 61% aa sequence identity with mouse and rat Caspase-1.
Additional Caspase-1 Products
Product Documents for Human Caspase-1 Antibody
Product Specific Notices for Human Caspase-1 Antibody
For research use only
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