Human Caspase-1 Antibody

Western Blot Shows Human Caspase-1 Specificity by Using Knockout Cell Line.

Western blot shows lysates of A431 human epithelial carcinoma parental cell line and Caspase‑1 knockout A431 cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Caspase-1 Monoclonal Antibody (Catalog # MAB62153) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Caspase-1 at approximately 48 kDa (as indicated) in the parental A431 cell line, but is not detectable in knockout A431 cell line. GAPDH (2275-PC-100) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Human Caspase-1 ELISA Standard Curve.

Recombinant Human Caspase-1 protein was serially diluted 2-fold and captured by Mouse Anti-Human Caspase-1 Monoclonal Antibody (MAB62151) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human Caspase-1 Monoclonal Antibody (Catalog # MAB62153) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).