Human Caspase-3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7071
Recombinant Monoclonal Antibody.
Key Product Details
Validated by
Orthogonal Validation, Biological Validation
Species Reactivity
Human
Applications
Dual RNAscope ISH-IHC Compatible, Immunocytochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1112I
Product Specifications
Immunogen
KLH-conjugated human Caspase-3 synthetic peptide
CRGTELDCGIETD
Accession # P42574
CRGTELDCGIETD
Accession # P42574
Specificity
Detects the active
form and p17 subunit of Caspase-3 in direct ELISAs, with less than 5%
cross-reactivity with the precursor form observed.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Caspase-3 Antibody
Caspase‑3 in Jurkat Human Cell Line.
Caspase-3 was detected in immersion fixed Jurkat human acute T cell leukemia cell line treated with Staurosporine using Rabbit Anti-Human Caspase-3 Monoclonal Antibody (Catalog # MAB7071) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Caspase-3 in Human Colon Using Dual RNAscope®ISH and IHC.
Caspase-3 mRNA was detected (top image) in formalin-fixed paraffin-embedded tissue sections of human colon probed with ACD RNAScope®Probe (Catalog # 436561) and stained using ACD RNAscope®2.5 HD Duplex Detection Reagents (red, Catalog # 322500). Adjacent tissue section (bottom image) was processed for immunohistochemistry using R&D Systems Rabbit Anti-Human Caspase-3 Monoclonal Antibody (Catalog# MAB7071) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC001) and DAB chromogen (brown). Tissues were counterstained with hematoxylin (blue).Applications for Human Caspase-3 Antibody
Application
Recommended Usage
Dual RNAscope ISH-IHC Compatible
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon
Sample: Immersion fixed paraffin-embedded sections of human colon
Immunocytochemistry
1-25 µg/mL
Sample: Immersion fixed Jurkat human acute T cell leukemia cell line treated with Staurosporine
Sample: Immersion fixed Jurkat human acute T cell leukemia cell line treated with Staurosporine
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB7071 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Caspase-3
Alternate Names
Apopain, CASP3, Caspase3, CPP32, LICE-1, YAMA
Gene Symbol
CASP3
UniProt
Additional Caspase-3 Products
Product Documents for Human Caspase-3 Antibody
Product Specific Notices for Human Caspase-3 Antibody
For research use only
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