Human Cathepsin D Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1014
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 185111
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cathepsin D
Leu21-Leu412
Accession # P07339
Leu21-Leu412
Accession # P07339
Specificity
Detects human Cathepsin D in direct ELISAs and Western blots. It recognizes both the pro and mature forms of recombinant human (rh) Cathepsin D. In direct ELISAs, no cross-reactivity with rhCathepsin A, rhCathepsin B, rhCathepsin C, rhCathepsin L, rhCathepsin O, rhCathepsin S, rhCathepsin Z, or recombinant mouse Cathepsin D is observed. In Western blots, 100% cross-reactivity with rhCathepsin E and rmCathepsin D is observed and no cross-reactivity with rhBACE-1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Cathepsin D Antibody
Detection of Human Cathepsin D by Western Blot.
Western blot shows lysates of PANC-1 human pancreatic carcinoma cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Cathepsin D Monoclonal Antibody (Catalog # MAB1014) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Cathepsin D at approximately 28 and 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cathepsin D in Human Prostate Cancer Tissue.
Cathepsin D was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-Human Cathepsin D Monoclonal Antibody (Catalog # MAB1014) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cell cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Human Cathepsin D by Simple WesternTM.
Simple Western lane view shows lysates of PANC‑1 human pancreatic carcinoma cell line and MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. Specific bands were detected for Cathepsin D at approximately 36 and 52-57 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human Cathepsin D Monoclonal Antibody (Catalog # MAB1014) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human Cathepsin D Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue
Simple Western
2 µg/mL
Sample: PANC‑1 human pancreatic carcinoma cell line and MCF‑7 human breast cancer cell line
Sample: PANC‑1 human pancreatic carcinoma cell line and MCF‑7 human breast cancer cell line
Western Blot
0.2 µg/mL
Sample: PANC‑1 human pancreatic carcinoma cell line and MCF‑7 human breast cancer cell line
Sample: PANC‑1 human pancreatic carcinoma cell line and MCF‑7 human breast cancer cell line
Reviewed Applications
Read 4 reviews rated 3.8 using MAB1014 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cathepsin D
Cathepsin D is a lysosomal aspartic protease of the pepsin family (1). Human cathepsin D is synthesized as a precursor protein, consisting of a signal peptide (aa 1-18), a propeptide (aa 19-64), and a mature chain (aa 65‑412) (2‑4). The mature chain can be processed further to the light (aa 65‑161) and heavy (aa 169‑412) chains. It is expressed in most cells and overexpressed in breast cancer cells (5). It is a major enzyme in protein degradation in lysosomes, and also involved in the presentation of antigenic peptides. Mice deficient in this enzyme showed a progressive atrophy of the intestinal mucosa, a massive destruction of lymphoid organs, and a profound neuronal ceroid lipofucinosis, indicating that cathepsin D is essential for proteolysis of proteins regulating cell growth and tissue homeostasis (6). Cathepsin D secreted from human prostate carcinoma cells are responsible for the generation of angiostatin, a potent endogeneous inhibitor of angiogenesis (6).
References
- Conner et al. in Handbook of Proteolytic Enzymes Barrett (1998) Academic Press, San Diego, p. 828.
- Faust, et al. (1985) Proc. Natl. Acad. Sci. USA 82:4910.
- Westley and May (1987) Nucl. Acid Res. 15:3773.
- Redecker, et al. (1991) DNA Cell Biol. 10:423.
- Rochefort, et al. (2000) Clin. Chim. Acta. 291:157.
- Tsukuba, et al. (2000) Mol. Cells 10:601.
Alternate Names
CTSD
Gene Symbol
CTSD
UniProt
Additional Cathepsin D Products
Product Documents for Human Cathepsin D Antibody
Product Specific Notices for Human Cathepsin D Antibody
For research use only
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