Detects human CCL19/MIP-3 beta in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 20, 21, 22, 23, 24, 25, recombinant mouse CCL2, 3, 4, 5, 6, 7, 9, 11, 12, 21, 22, or 25 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human CCL19/MIP-3 beta Antibody

CCL19/MIP-3 beta in Human Tonsil.

CCL19/MIP-3 beta was detected in immersion fixed paraffin-embedded sections of human tonsil using 25 µg/mL Mouse Anti-Human CCL19/ MIP-3 beta Monoclonal Antibody (Catalog # MAB361) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

CCL19/MIP-3 beta in Human PBMCs.

CCL19/MIP-3 beta was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Mouse Anti-Human CCL19/MIP-3 beta Monoclonal Anti-body (Catalog # MAB361) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of CCL19/MIP‑3 beta in Human Dendritic Cells by Flow Cytometry.

Human monocyte-derived dendritic cells were stained with Mouse Anti-Human CCL19/MIP-3 beta Monoclonal Antibody (Catalog # MAB361, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).

Applications for Human CCL19/MIP-3 beta Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human monocyte-derived dendritic cells

Western Blot

1 µg/mL
Sample: Recombinant Human CCL19/MIP-3 beta (Catalog # 361-MI)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB361 in the following applications:

Immunohistochemistry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

For liquid material, refer to CoA for concentration.

Formulation

Supplied as a 0.2 μm filtered solution in Tris and Acetic Acid. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.

Background: CCL19/MIP-3 beta

CCL19, also known as MIP-3 beta and ELC (EBI1-Ligand Chemokine), is a 77 amino acid (aa) beta chemokine that is distantly related to other beta chemokines (20-30% aa sequence identity). The gene for MIP-3 beta has been mapped to chromosome 9p13 rather than chromosome 17 where the genes for many human beta chemokines are clustered. MIP-3 beta is constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix and spleen). The expression of MIP-3 beta is
down‑regulated by the anti-inflammatory cytokine IL-10. Recombinant MIP-3 beta is chemotactic for cultured human lymphocytes. MIP-3 beta is a ligand for CCR7 (previously referred to as the Epstein-Barr virus-induced gene 1 (EBI1) orphan receptor), a chemokine receptor that is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR7 is strongly up-regulated in B cells infected with Epstein-Barr virus and T cells infected with herpesvirus 6 or 7.

References

Yoshida, R. et al. (1997) J. Biol. Chem. 272:13803.

Alternate Names

ELC, MIP-3 beta, MIP3 beta

Entrez Gene IDs

6363 (Human); 24047 (Mouse); 362506 (Rat)

Gene Symbol

CCL19

UniProt

Q99731.1

Additional CCL19/MIP-3 beta Products

Product Documents for Human CCL19/MIP-3 beta Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human CCL19/MIP-3 beta Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CCL19/MIP-3 beta Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB361

Key Product Details

Species Reactivity

Validated:

Cited:

Applications