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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 24822

Product Specifications

Immunogen

E. coli-derived recombinant human CCL2/JE/MCP-1
Accession # P13500

Specificity

Detects human CCL2/JE/MCP-1 in Western blots. Does not cross-react with recombinant human CCL1, 3, 4, 5, 7, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 9,10, MIP-1 gamma, 11, 12, 17, 19, 20, 22, 24, 25, or recombinant rat CCL20.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CCL2/JE/MCP-1 Alexa Fluor® 488-conjugated Antibody

Detection of CCL2/JE/MCP-1 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of CCL2/JE/MCP-1 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) treated with 250 ng/mL LPS overnight were stained with Mouse Anti-Human CCL2/JE/MCP-1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC279G) and Mouse Anti-Human CD11c APC-conjugated Monoclonal Antibody (Catalog # FAB1777A). Quadrant markers were set based on control antibody staining (Catalog # IC002G). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human CCL2/JE/MCP-1 Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

5 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with LPS were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCL2/JE/MCP-1

CCL2 is a beta-chemokine that is also known as Monocyte Chemotactic Protein 1 (MCP-1). It is produced by a variety of cell types and is chemotactic for monocytes and basophils. MCP-1 exists in equilibrium as either a monomer or homodimer, and forms a heterodimer with multiple chemokines. Human and mouse CCL2 share 58% amino acid sequence identity.

Alternate Names

GDCF-2, HC11, HSMCR30, MCAF, MCP-1, SMC-CF

Entrez Gene IDs

6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)

Gene Symbol

CCL2

UniProt

Additional CCL2/JE/MCP-1 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CCL2/JE/MCP-1 Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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