Detects human CCL2/JE/MCP-1 in Western blots. Does not cross-react with recombinant human (rh) CCL1, 3, 4, 5, 7, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, rmCCL1, 2, 3, 4, 5, 6, 7, 9/10MIP-1 gamma, 11, 12, 17, 19, 20, 22, 24, 25, or rrCCL20.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CCL2/JE/MCP-1 Antibody

Detection of Recombinant Human CCL2/JE/MCP‑1 by Western Blot.

Western blot shows 25 ng of Recombinant Human CCL2/JE/MCP-1 (Catalog # 279-MC), Recombinant Human CCL8/ MCP-2 (Catalog # 281-CP), Recombinant Human CCL7/MCP-3/MARC (Catalog # 282-P3), Recombinant Human CCL13/ MCP-4 (Catalog # 327-P4), Recombinant Human CCL11/Eotaxin (Catalog # 320-EO), Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE), and Recombinant Rat CCL2/JE/MCP-1 (Catalog # 3144-JE). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human CCL2/JE/MCP-1 Monoclonal Antibody (Catalog # MAB279) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CCL2/JE/ MCP-1 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Chemotaxis Induced by CCL2/JE/MCP‑1 and Neutralization by Human CCL2/JE/MCP‑1 Antibody.

Recombinant Human CCL2/JE/ MCP-1 (Catalog # 279-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL2/JE/ MCP-1 (0.15 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL2/JE/ MCP-1 Monoclonal Antibody (Catalog # MAB279). The ND₅₀ is typically 0.9-4.5 µg/mL.

Applications for Human CCL2/JE/MCP-1 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human CCL2/JE/MCP-1 (Catalog # 279-MC)

Neutralization

Measured by its ability to neutralize CCL2/JE/MCP-1-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR2A. The Neutralization Dose (ND₅₀) is typically 0.9-4.5 µg/mL in the presence of 0.15 µg/mL Recombinant Human CCL2/JE/MCP-1.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 5 reviews rated 4.6 using MAB279 in the following applications:

ELISA (3 Reviews)
MSD assay (1 Review)
Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL2/JE/MCP-1

CCL2 is a beta-chemokine that is also known as monocyte chemotactic protein 1 (MCP-1). It is produced by a variety of cell types and is chemotactic for monocytes.

Alternate Names

GDCF-2, HC11, HSMCR30, MCAF, MCP-1, SMC-CF

Entrez Gene IDs

6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)

Gene Symbol

CCL2

Additional CCL2/JE/MCP-1 Products

Product Documents for Human CCL2/JE/MCP-1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human CCL2/JE/MCP-1 Antibody

For research use only

Citations
Reviews

Human CCL2/JE/MCP-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB279

Key Product Details

Species Reactivity

Validated:

Cited:

Applications