Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 21445

View all CCL5/RANTES Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CCL5/RANTES
Accession # P13501

Specificity

Detects human CCL5/RANTES in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with other chemokines, including recombinant human CCL2, 3, 4,  recombinant mouse (rm) CCL3, and rmCCL4.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CCL5/RANTES APC-conjugated Antibody

Detection of CCL5/RANTES antibody in NS0 Mouse Cell Line Transfected with Human CCL5/RANTES antibody by Flow Cytometry.

Detection of CCL5/RANTES in NS0 Mouse Cell Line Transfected with Human CCL5/RANTES by Flow Cytometry.

NS0 mouse myeloma cell line transfected with human CCL5/RANTES was stained with Mouse Anti-Human CCL5/RANTES APC-conjugated Monoclonal Antibody (Catalog # IC278A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human CCL5/RANTES APC-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: NS0 mouse myeloma cell line transfected with human CCL5/RANTES fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CCL5/RANTES

RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted) is a member of the beta (C-C) chemokine subfamily and is now designated CCL5. It binds and activates the chemokine receptors CCR1, 3 and 5, and is reported to be a ligand for GPR75. CCL5 contains O-linked glycosylation and undergoes N-terminal processing by MMP-13. CCL5 homooligomers likely exist, and heterodimers with CXCL4 and CCL2 have been noted. Functionally, CCL5 promotes the migration of CD8+ T cells, macrophages, and eosinophils, and induces the formation of chemokine-activated killer cells. Human and mouse CCL5 share 84% sequence identity.

Alternate Names

RANTES, SISd

Entrez Gene IDs

6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)

Gene Symbol

CCL5

UniProt

P13501

Additional CCL5/RANTES Products

Product Documents for Human CCL5/RANTES APC-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CCL5/RANTES APC-conjugated Antibody

For research use only

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