Human CCL7/MCP-3/MARC Antibody
R&D Systems, part of Bio-Techne | Catalog # AF-282-NA
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Neutralization, Western Blot
Cited:
Neutralization
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CCL7/MCP‑3/MARC (R&D Systems, Catalog # 282-P3)
Gln34-Leu109
Accession # Q7Z7Q8
Gln34-Leu109
Accession # Q7Z7Q8
Specificity
Detects human CCL7/MCP‑3/MARC in direct ELISAs and Western blots. In these formats, less than 20% cross-reactivity with recombinant human (rh) Eotaxin is observed and less than 5% cross-reactivity with rhMCP-2, rhMCP-1, and recombinant mouse JE is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CCL7/MCP-3/MARC Antibody
Chemotaxis Induced by CCL7/MCP-3 and Neutralization by Human CCL7/MCP-3 Antibody.
Recombinant Human CCL7/MCP-3 (Catalog # 282-P3) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL7/MCP-3 (1 µg/mL) is neutralized (green line) by increasing concentrations of Human CCL7/MCP-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-282-NA). The ND50 is typically 10-30 µg/mL.Applications for Human CCL7/MCP-3/MARC Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human CCL7/MCP-3/MARC (Catalog # 282-P3)
Sample: Recombinant Human CCL7/MCP-3/MARC (Catalog # 282-P3)
Neutralization
Measured by its ability to neutralize CCL7/MCP-3/MARC-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 10-30 µg/mL in the presence of 1 µg/mL Recombinant Human CCL7/MCP-3/MARC.
Reviewed Applications
Read 3 reviews rated 5 using AF-282-NA in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL7/MCP-3/MARC
MCP-2 and CCL7 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and CCL7 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. CCL7 also shares 58% amino acid identity with MCP-2.
CCL7 cDNA encodes a 99 amino acid residue precursor protein from which the N-terminal 23 amino acid residues are cleaved to generate the 76 amino acid residue mature CCL7. Mature CCL7 contains a potential N-linked and several possible O-linked glycosylation sites.
Similar to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.
Alternate Names
MARC, MCP-3
Gene Symbol
CCL7
UniProt
Additional CCL7/MCP-3/MARC Products
Product Documents for Human CCL7/MCP-3/MARC Antibody
Product Specific Notices for Human CCL7/MCP-3/MARC Antibody
For research use only
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